Rice blast disease, caused by Magnaporthe oryzae, results in an extensive loss of rice productivity. Previously, we identified a novel M. oryzae secreted protein, termed MSP1 which causes cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. Here, we report the transcriptome profile of MSP1-induced response in rice, which led to the identification of 21,619 genes, among which 4,386 showed significant changes (P < 0.05 and fold change > 2 or < 1/2) in response to exogenous MSP1 treatment. Functional annotation of differentially regulated genes showed that the suppressed genes were deeply associated with photosynthesis, secondary metabolism, lipid synthesis, and protein synthesis, while the induced genes were involved in lipid degradation, protein degradation, and signaling. Moreover, expression of genes encoding receptor-like kinases, MAPKs, WRKYs, hormone signaling proteins and pathogenesis-related (PR) proteins were also induced by MSP1. Mapping these differentially expressed genes onto various pathways revealed critical information about the MSP1-triggered responses, providing new insights into the molecular mechanism and components of MSP1-triggered PTI responses in rice.

中文翻译：

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水稻叶片的转录组学分析揭示了对稻瘟病菌MSP1响应的关键变化。

由稻瘟病菌引起的稻瘟病导致稻米生产力的大量丧失。以前，我们确定了一种新颖的米曲霉分泌蛋白，称为MSP1，它引起水稻中的细胞死亡和病原体相关分子模式（PAMP）触发的免疫（PTI）反应。在这里，我们报告了MSP1诱导的水稻反应的转录组图谱，从而鉴定了21,619个基因，其中4,386个基因显示了显着变化（P<0.05且倍数变化> 2或<1/2）响应于外源MSP1处理。差异调节基因的功能注释显示，被抑制的基因与光合作用，次级代谢，脂质合成和蛋白质合成密切相关，而诱导的基因与脂质降解，蛋白质降解和信号传导有关。此外，MSP1还可诱导编码受体样激酶，MAPK，WRKY，激素信号蛋白和发病相关（PR）蛋白的基因的表达。将这些差异表达的基因定位到各种途径上揭示了有关MSP1触发的应答的关键信息，从而为水稻中MSP1触发的PTI应答的分子机制和成分提供了新见解。