Previous studies have shown that dissolved substances in some natural hot springs have analgesic/anti-nociceptive and anti-inflammatory actions. However, the mechanisms underlying how such dissolved substances exert these actions are not fully understood. In the present study on mice, we examined the analgesic/anti-nociceptive and anti-inflammatory properties of a mineral cream containing natural hot spring ingredients. The anti-nociceptive effects of the mineral cream were assessed by using the von Frey test. Application of the mineral cream to the hind paw of mice produced a significant anti-nociceptive effect compared to control. The anti-nociceptive effects of the mineral cream were also assessed following the injection of complete Freund's adjuvant (CFA) into the hind paws of mice after pre-treatment for one or four weeks with the mineral cream. Histological experiments with light microscopy showed that the mineral cream did not reduce inflammation caused by the CFA treatment. In addition, the mineral cream did not inhibit oxidative stress as evidenced by increased levels of oxidative metabolites (d-ROMs) and biological anti-oxidant potential (BAP). These results suggest that the mineral cream does not exert a protective effect against inflammation, and that the constituents of the mineral cream may produce their anti-nociceptive effects transdermally via different mechanisms including the nervous system.

中文翻译：

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含有天然温泉矿物质的矿物质奶油的镇痛作用，可用于皮肤。

先前的研究表明，某些天然温泉中的溶解物质具有止痛/抗伤害和消炎作用。但是，尚未完全了解这种溶解物质如何发挥这些作用的机制。在对小鼠的本研究中，我们检查了含有天然温泉成分的矿物质乳膏的镇痛/抗伤害感受和抗炎特性。通过使用冯·弗雷（von Frey）试验评估了矿物质乳膏的抗伤害感受作用。与对照相比，将矿物乳膏施用于小鼠的后爪产生了显着的抗伤害感受作用。注射完整的Freund'之后，还评估了矿物乳膏的抗伤害感受作用 矿物乳膏预处理小鼠一到四周后，将其佐剂（CFA）注入小鼠后爪。光学显微镜的组织学实验表明，矿物质霜不能减轻CFA治疗引起的炎症。此外，矿物质乳膏不抑制氧化应激，这是由氧化代谢物（d-ROM）和生物抗氧化电位（BAP）含量增加所证明的。这些结果表明，矿物质乳膏没有对炎症的保护作用，并且矿物质乳膏的成分可以通过包括神经系统在内的不同机制经皮产生抗伤害感受的作用。如氧化代谢产物（d-ROMs）和生物抗氧化电位（BAP）的增加所证明的那样，矿物质乳膏不抑制氧化应激。这些结果表明，矿物质乳膏没有对炎症的保护作用，并且矿物质乳膏的成分可以通过包括神经系统在内的不同机制经皮产生抗伤害感受的作用。如氧化代谢物（d-ROMs）和生物抗氧化电位（BAP）的增加所证明的那样，矿物质霜不能抑制氧化应激。这些结果表明，矿物质乳膏没有对炎症的保护作用，并且矿物质乳膏的成分可以通过包括神经系统在内的不同机制经皮产生抗伤害感受的作用。