Although model species have proven to be crucial for developmental biology, the evo‐devo approach requires a broader picture across phylogeny. Herein, we try to expand the range of studied annelids by presenting a transcriptome of Typosyllis antoni as a tool for the study of developmental and evolutionary processes in Syllidae. Moreover, we provide homologs of the stem‐cell markers vasa, piwi, and nanos, and investigate their expression patterns in gamete‐producing individuals for the first time in this group. We found no expression in females, while there is a distinct expression pattern in males. Based on this data, we argue that spermatogenesis starts in the gonads and finishes in the coelomic cavity, and it occurs simultaneously in a large number of segments. Surprisingly, no expression of the stem‐cell markers was found in the segment addition zone of these reproducing animals (stolonizing). Preliminary explanations like a lack of growth during stolonization, or the absence of a common genetic program between germ and somatic stem cells, are discussed. Finally, no reservoir of primordial cells has been detected, suggesting a possible epigenic origin of the Primordial Germ Cells of this species, though this hypothesis needs to be further investigated.

中文翻译：

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vasa，piwi和nanos的表达在配子发生期间在安东尼氏拟南芥（Annelida，Syllidae）。

尽管已证明模型物种对于发育生物学至关重要，但进化论方法需要更广泛的系统发育图。在这里，我们试图通过提出安东尼奥Typosyllis antoni的转录组作为研究锡兰（Syllidae）发育和进化过程的工具，来扩大已研究过的Annelids的范围。此外，我们提供干细胞标记vasa，piwi和nanos的同源物，并首次在该组中研究其在配子产生个体中的表达模式。我们发现雌性没有表达，而雄性有明显的表达模式。基于这些数据，我们认为精子发生在性腺中开始，并在腔腔中完成，并且在许多节段中同时发生。出乎意料的是，在这些繁殖动物的节段添加区中没有发现干细胞标记的表达（定殖）。讨论了初步的解释，例如在stolonization过程中缺乏生长，或在生殖细胞和体干细胞之间缺乏共同的遗传程序。最终，未检测到原始细胞的储库，表明该物种的原始生殖细胞可能是表观遗传起源，