The green-lipped mussel, Perna viridis, is considered to be an ideal indicator for marine environmental pollution. Dichlorodiphenyltrichloroethane (DDT), a typical persistent organic pollutant, is extensively distributed in marine environments. However, little is known about the toxic effects of DDT on the embryo of marine animals, particularly in marine bivalves. Using next-generation sequencing technology, we studied P. viridis embryo after DDT stress at the transcriptome level. A total of 99 202 unigenes were obtained based on the 2383 bp of unigene N50. These differentially expressed genes (DEGs) participated in the various molecular pathways of biological effects, including oxidative stress, detoxification, innate immunity and neurobehavioral disease. Quantitative real-time PCR was performed to verify the mRNA expression of several genes identified by differential gene expression (DGE) analysis. The results indicated that DDT was in induced a dose-dependent manner in the embryo of P. viridis, and most genes involved in oxidative stress and detoxification were up-regulated by DDT exposure; however, the immunity-related genes were down-regulated, except the genes involved in phagocytosis. Gene expression changes in embryo from P. viridis provide a preliminary basis to better understand the molecular toxic response mechanisms of embryo to DDT.

中文翻译：

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暴露于二氯二苯基三氯乙烷（DDT）的贻贝绿色贻贝的胚胎中的差异基因响应。

唇绿色的贻贝Perna viridis被认为是海洋环境污染的理想指标。二氯二苯基三氯乙烷（DDT）是一种典型的持久性有机污染物，广泛分布在海洋环境中。然而，关于滴滴涕对海洋动物，特别是海洋双壳类动物的胚胎的毒性作用知之甚少。使用下一代测序技术，我们在转录组水平上研究了DDT胁迫后的绿色假单胞菌胚胎。基于单基因N50的2383 bp，获得了总共99 202个单基因。这些差异表达基因（DEGs）参与了各种生物效应的分子途径，包括氧化应激，排毒，先天免疫和神经行为疾病。进行定量实时PCR以验证通过差异基因表达（DGE）分析鉴定的几种基因的mRNA表达。结果表明，滴滴涕在绿色假单胞菌的胚胎中呈剂量依赖性，大部分暴露于氧化应激和排毒的基因都通过滴滴涕上调。然而，除了与吞噬作用有关的基因外，与免疫相关的基因被下调。绿色假单胞菌胚胎中的基因表达变化为更好地了解胚胎对滴滴涕的分子毒性反应机制提供了初步的基础。除与吞噬作用有关的基因外，与免疫相关的基因被下调。绿色假单胞菌胚胎中的基因表达变化为更好地了解胚胎对滴滴涕的分子毒性反应机制提供了初步的基础。除与吞噬作用有关的基因外，与免疫相关的基因被下调。绿色假单胞菌胚胎中的基因表达变化为更好地了解胚胎对滴滴涕的分子毒性反应机制提供了初步的基础。