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Quantification of Cerenkov Luminescence Imaging (CLI) Comparable With 3-D PET Standard Measurements.
Molecular Imaging ( IF 2.8 ) Pub Date : 2018-07-26 , DOI: 10.1177/1536012118788637
Frezghi Habte 1 , Arutselvan Natarajan 1 , David S Paik 1 , Sanjiv Sam Gambhir 1
Affiliation  

Cerenkov luminescence imaging (CLI) is commonly performed using two-dimensional (2-D) conventional optical imaging systems for its cost-effective solution. However, quantification of CLI comparable to conventional three-dimensional positron emission tomography (PET) is challenging using these systems due to both the high attenuation of Cerenkov radiation (CR) on mouse tissue and nonexisting depth resolution of CLI using 2-D imaging systems (2-D CLI). In this study, we developed a model that estimates effective tissue attenuation coefficient and corrects the tissue attenuation of CLI signal intensity independent of tissue depth and size. To evaluate this model, we used several thin slices of ham as a phantom and placed a radionuclide (89Zr and 64Cu) inside the phantom at different tissue depths and sizes (2, 7, and 12 mm). We performed 2-D CLI and MicroPET/CT (Combined small animal PET and Computed Tomography (CT)) imaging of the phantom and in vivo mouse model after administration of 89Zr tracer. Estimates of the effective tissue attenuation coefficient (μeff) for 89Zr and 64Cu were ∼2.4 and ∼2.6 cm-1, respectively. The computed unit conversion factor to %ID/g from 2-D CLI signal was 2.74 × 10-3 μCi/radiance estimated from phantom study. After applying tissue attenuation correction and unit conversion to the in vivo animal study, an average quantification difference of 10% for spleen and 35% for liver was obtained compared to PET measurements. The proposed model provides comparable quantification accuracy to standard PET system independent of deep tissue CLI signal attenuation.

中文翻译:

与3-D PET标准测量相当的Cerenkov发光成像(CLI)定量。

切伦科夫(Cerenkov)发光成像(CLI)通常使用二维(2-D)常规光学成像系统执行,以提供经济高效的解决方案。但是,由于在小鼠组织上切伦科夫辐射(CR)的高衰减和使用2-D成像系统的CLI的深度分辨率都不高,使用这些系统对CLI进行定量分析(与传统的三维正电子发射断层扫描(PET)相比)具有挑战性( 2-D CLI)。在这项研究中,我们开发了一个模型,该模型可估计有效的组织衰减系数并校正CLI信号强度的组织衰减,而与组织的深度和大小无关。为了评估该模型,我们使用了几个火腿薄片作为人体模型,并在人体内部以不同的组织深度和大小(2、7和12 mm)放置了放射性核素(89Zr和64Cu)。服用89Zr示踪剂后,我们对体模和体内小鼠模型进行了二维CLI和MicroPET / CT(小动物PET和计算机断层扫描(CT)组合成像)。89Zr和64Cu的有效组织衰减系数(μeff)分别约为2.4和2.6 cm-1。从幻像研究估计的从2-D CLI信号到%ID / g的计算单位转换因子为2.74×10-3μCi/辐射。将组织衰减校正和单位转换应用于体内动物研究后,与PET测量相比,脾脏的平均定量差异为10%,肝脏的平均定量差异为35%。所提出的模型提供了与标准PET系统相当的定量准确性,而与深层组织CLI信号衰减无关。
更新日期:2019-11-01
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