Host range and cross-infectivity studies are important for identifying rhizobial strains with potential for use as inoculants. In this study, 10 native soybean rhizobia isolated from Mozambican and South African soils were evaluated for host range, symbiotic effectiveness and ability to induce high rates of photosynthesis leading to enhanced plant growth in cowpea (Vigna unguiculata L. Walp.), Bambara groundnut (Vigna subterranean L. Verdc.), Kersting’s groundnut (Macrotyloma geocarpum Harm) and soybean (Glycine max L. Merr). The test isolates had different growth rates and colony sizes. Molecular analysis based on enterobacterial repetitive intergenic consensus (ERIC)-PCR revealed high genetic diversity among the test isolates. The results further showed that isolate TUTLBC2B failed to elicit nodulation in all test plants, just as TUTNSN2A and TUTDAIAP3B were also unable to nodulate cowpea, Kersting’s bean and Bambara groundnut. Although the remaining strains formed ineffective nodules on cowpea and Kersting’s bean, they induced effective nodules on Bambara groundnut and the two soybean genotypes. Bacterial stimulation of nodule numbers, nodule dry weights and photosynthetic rates was generally greater with isolates TUTRSRH3A, TUTM19373A, TUTMCJ7B, TUTRLR3B and TUTRJN5A. As a result, these isolates elicited significantly increased accumulation of biomass in shoots and whole plants of Bambara groundnut and the two soybean genotypes. Whole-plant symbiotic nitrogen (N) of soybean and Bambara groundnut was highest for the commercial strains CB756 and WB74, as well as for TUTRLR3B, TUTMCJ7B and TUTRSRH3A, suggesting that the three native rhizobial isolates have potential for use as inoculants.

中文翻译：

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评估从莫桑比克和南非土壤中分离的天然大豆根瘤菌诱导的寄主范围，共生有效性和光合速率。

寄主范围和交叉感染性研究对于鉴定有潜力用作接种剂的根瘤菌菌株很重要。在这项研究中，评估了从莫桑比克和南非土壤中分离的10种天然大豆根瘤菌的寄主范围，共生有效性以及诱导高光合作用的能力，从而增强了cow豆（Vigna unguiculata L. Walp。），班巴拉花生（Vigna地下L.Verdc。），Kersting花生（Macrotyloma geocarpum Harm）和大豆（Glycine maxL. Merr）。测试分离株具有不同的生长速率和菌落大小。基于肠细菌重复基因间共有（ERIC）-PCR的分子分析显示，测试分离株之间具有很高的遗传多样性。结果进一步表明，分离株TUTLBC2B未能在所有测试植物中引起结瘤，就像TUTNSN2A和TUTDAIAP3B也无法结瘤cow豆，克ersting豆和Bambara花生一样。尽管其余的菌株在cow豆和克星廷豆上形成无效的根瘤，但它们对班巴拉花生和两种大豆基因型诱导了有效的根瘤。细菌分离株TUTRSRH3A，TUTM19373A，TUTMCJ7B，TUTRLR3B和TUTRJN5A对结节数量，结节干重和光合速率的细菌刺激作用通常更大。因此，这些分离物引起了班巴拉花生和两种大豆基因型的芽和整个植物中生物量的积累显着增加。商业菌株CB756和WB74以及TUTRLR3B，TUTMCJ7B和TUTRSRH3A的大豆和班巴拉花生的全植物共生氮（N）最高，表明这三种天然的根瘤菌分离物具有用作孕育剂的潜力。