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Nuclear dynamics in the homothallic ascomycete Sordaria fimicola.
Botanical Studies ( IF 3.4 ) Pub Date : 2018-07-05 , DOI: 10.1186/s40529-018-0233-y
Kulsumpun Krobanan,Wei-Chiang Shen

BACKGROUND The coprophilous ascomycete Sordaria fimicola usually reproduces sexually. Sexual differentiation in S. fimicola is accompanied by cellular and morphological changes, followed by multicellular tissue development to complete the sexual cycle. Although the morphological features of the sexual reproductive structure in S. fimicola have been well characterized, little is known about the nuclear dynamics and organization during these processes. Therefore, in this study, we successfully developed an Agrobacterium-mediated protoplast transformation protocol and generated histone H2B-mCherry-labeled S. fimicola strains. The life cycle of S. fimicola begins with germination of the ascospore and ends with the formation and discharge of new ascospores from the mature black sexual fruiting bodies, the so-called perithecia. The nuclear dynamics of the fluorescently labeled strains were examined during ascospore germination, hyphal elongation, and hyphal fusion using fluorescent microscopy. RESULTS Live imaging revealed that the nuclei in the germlings and fusion hyphae during the pre-contact interaction are located adjacent to the tip. CONCLUSIONS This is the first report of the application of a fluorescence labeling technique in S. fimicola. This application will help researchers gain a better understanding of nuclear distribution and investigate the protein-protein interaction networks during fruiting body formation for advanced molecular genetic studies in S. fimicola.

中文翻译:

同形子囊菌Sordaria fimicola中的核动力学。

背景技术伴生的子囊菌Sordaria fimicola通常有性繁殖。Fimicola中的性别分化伴随着细胞和形态的变化,随后是多细胞组织的发育以完成性周期。尽管已很好地鉴定了线虫链球菌有性生殖结构的形态特征,但对这些过程中的核动力学和组织了解甚少。因此,在这项研究中,我们成功地开发了农杆菌介导的原生质体转化方案,并产生了组蛋白H2B-mCherry标记的S. fimicola菌株。Fimicola的生命周期始于子囊孢子的萌芽,并从成熟的黑色有性子实体(所谓的皮膜组织)形成和排出新的子囊孢子而结束。使用荧光显微镜检查了子囊萌发,菌丝伸长和菌丝融合过程中荧光标记菌株的核动力学。结果实时成像显示,在接触前相互作用期间,胚芽和融合菌丝中的核位于尖端附近。结论这是荧光标记技术在S. fimicola中的首次报道。该应用程序将帮助研究人员更好地了解核分布,并研究子实体形成过程中的蛋白质-蛋白质相互作用网络,以进行金黄色葡萄球菌的高级分子遗传学研究。结果实时成像显示,在接触前相互作用期间,胚芽和融合菌丝中的核位于尖端附近。结论这是荧光标记技术在S. fimicola中的首次报道。该应用程序将帮助研究人员更好地了解核分布,并研究子实体形成过程中的蛋白质-蛋白质相互作用网络,以进行金黄色葡萄球菌的高级分子遗传学研究。结果实时成像显示,在接触前相互作用期间,胚芽和融合菌丝中的核位于尖端附近。结论这是荧光标记技术在S. fimicola中的首次报道。该应用程序将帮助研究人员更好地了解核分布,并研究子实体形成过程中的蛋白质-蛋白质相互作用网络,以进行金黄色葡萄球菌的高级分子遗传学研究。
更新日期:2019-11-01
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