BACKGROUND Gastric mucosal integrity is maintained through achieving a balance between potentially detrimental and protective factors. This study aimed to evaluate the effects of Vigiis 101 powder, a product consisting of dried Lactobacillus paracasei subsp. paracasei NTU 101 bacteria, on intestinal bacterial flora and acute damage to the gastric mucosa induced by pylorus ligation. METHOD Acute gastric injury were induced by pylorus ligation. Rats were orally treated with the vehicle or 0.15, 0.30, 1.50 g/kg (0.5-, 1-, and 5-x) Vigiis 101 for 8 weeks. We analyzed the intestinal microflora, short-chain fatty acids, antioxidant enzyme levels and gastric lesion. RESULTS Daily treatment of rats with Vigiis 101 increased the levels of beneficial Bifidobacterium spp. and Lactobacillus spp., and reduced those of detrimental Clostridium perfringens and Enterobacteriaceae. Vigiis 101 treatment also markedly reduced gastric lesion areas by between 1.7 and 11.4%, and significantly increased the levels of the short-chain fatty acids (SCFAs): acetic acid, propionic acid, and butyric acid in cecal samples by 45-86%, 127-158%, and 20-211%, respectively, compared to non-treated controls. In the gastric mucosa of rats treated with Vigiis 101, the activity of superoxide dismutase and levels of prostaglandin E2 (PGE2) were enhanced, and the activity of lipid peroxidase reduced, relative to controls. CONCLUSION The up-regulation of PGE2 and SCFAs in the gastric mucosa is likely to stimulate mucus secretion and increase transmucosal resistance, thereby contributing to Vigiis 101-mediated protection from gastric mucosal lesions induced by pylorus ligation.

中文翻译：

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商业乳酸菌产品Vigiis 101对大鼠胃粘膜和肠道细菌菌群的有益作用。

背景技术通过实现潜在有害和保护因素之间的平衡来维持胃粘膜完整性。这项研究旨在评估Vigiis 101粉末的效果，该粉末由干燥的副干酪乳杆菌亚种组成。副干酪NTU 101细菌，对肠道细菌菌群和幽门结扎引起的胃粘膜急性损伤。方法幽门结扎术可引起急性胃损伤。用赋形剂或0.15、0.30、1.50 g / kg（0.5-，1-和5-x）Vigiis 101口服治疗大鼠8周。我们分析了肠道菌群，短链脂肪酸，抗氧化酶水平和胃部病变。结果每日用Vigiis 101大鼠处理可增加有益双歧杆菌属的水平。和乳杆菌属 减少有害的产气荚膜梭状芽胞杆菌和肠杆菌科细菌。Vigiis 101处理还可显着减少1.7至11.4％的胃部病变区域，并显着增加盲肠样品中的短链脂肪酸（SCFA）：乙酸，丙酸和丁酸的水平45-86％，与未处理的对照组相比，分别为127-158％和20-211％。与对照组相比，在用Vigiis 101治疗的大鼠的胃粘膜中，超氧化物歧化酶的活性和前列腺素E2（PGE2）的水平增强，脂质过氧化物酶的活性降低。结论胃粘膜中PGE2和SCFAs的上调可能刺激粘液分泌并增加跨粘膜抵抗力，