Insect pests such as Callosobruchus maculatus Fabricius and Plodia interpunctella Hübner cause substantial losses to grain during postharvest storage. In the last few years, hermetic storage technologies have been successfully used by smallholder farmers in Africa and Asia to protect their harvested grain against insect pests. Hermetic technologies owe much of their effectiveness to restricting oxygen availability to insects confined in the containers. There is a need to better understand the biology of specific storage insect pests and their responses to hypoxia. We employed a novel and non-invasive analytical technology, the OxySense 5250i, to measure oxygen levels in closed containers, and evaluated its effectiveness in measuring the total oxygen consumption of two insect pests during their development: C. maculatus and P. interpunctella. The total amount of oxygen consumed by C. maculatus during its larval development period determined with the OxySense apparatus was not different from that previously recorded using another instrument, the Mocon Pac Check 325 gas analyzer. Using the OxySense 5250i, we found that P. interpunctella consumes nearly three times as much oxygen per insect over its larval-to-adult developmental period compared to C. maculatus. Information on the lifetime oxygen consumption of insects provides relevant information to the effectiveness and ability of hermetic technologies to protect stored products against insect pests.

中文翻译：

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两种收获后害虫发育过程中的累积耗氧量：Callosobruchus maculatus Fabricius 和 Plodia interpunctella Hübner

Callosobruchus maculatus Fabricius 和 Plodia interpunctella Hübner 等害虫在收获后储存期间对谷物造成重大损失。在过去几年中，非洲和亚洲的小农成功地使用密封储存技术来保护他们收获的谷物免受虫害。密封技术的大部分有效性归功于限制容器中昆虫的氧气供应。有必要更好地了解特定储藏害虫的生物学及其对缺氧的反应。我们采用了一种新颖的非侵入性分析技术 OxySense 5250i 来测量密闭容器中的氧气含量，并评估其在测量两种害虫发育过程中总耗氧量的有效性：C. maculatus 和 P. interpunctella。使用 OxySense 设备确定的 C. maculatus 在其幼虫发育期间消耗的氧气总量与之前使用另一种仪器 Mocon Pac Check 325 气体分析仪记录的总量没有什么不同。使用 OxySense 5250i，我们发现与 C. maculatus 相比，P. interpunctella 在其幼虫到成虫发育期间每只昆虫消耗的氧气几乎是 C. maculatus 的三倍。关于昆虫终生耗氧量的信息为密封技术保护储存产品免受虫害的有效性和能力提供了相关信息。使用 OxySense 5250i，我们发现与 C. maculatus 相比，P. interpunctella 在其幼虫到成虫发育期间每只昆虫消耗的氧气几乎是 C. maculatus 的三倍。关于昆虫终生耗氧量的信息为密封技术保护储存产品免受虫害的有效性和能力提供了相关信息。使用 OxySense 5250i，我们发现与 C. maculatus 相比，P. interpunctella 在其幼虫到成虫发育期间每只昆虫消耗的氧气几乎是 C. maculatus 的三倍。关于昆虫终生耗氧量的信息为密封技术保护储存产品免受虫害的有效性和能力提供了相关信息。