Tomato chlorosis virus (ToCV) is a whitefly-transmitted and phloem-limited crinivirus. In 2013, severe interveinal chlorosis and bronzing on tomato leaves, known symptoms of ToCV infection, were observed in greenhouses in Korea. To identify ToCV infection in symptomatic tomato plants, RT-PCR with ToCV-specific primers was performed on leaf samples collected from 11 tomato cultivating areas where ToCV-like symptoms were observed in 2013 and 2014. About half of samples (45.18%) were confirmed as ToCV-infected, and the complete genome of 10 different isolates were characterized. This is the first report of ToCV occurring in Korea. The phylogenetic relationship and genetic variation among ToCV isolates from Korea and other countries were also analysed. When RNA1 and RNA2 are analysed separately, ToCV isolates were clustered into three groups in phylogenetic trees, and ToCV Korean isolates were confirmed to belong to two groups, which were geographically separated. These results suggested that Korean ToCV isolates originated from two independent origins. However, the RNA1 and RNA2 sequences of the Yeonggwang isolate were confirmed to belong to different groups, which indicated that ToCV RNA1 and RNA2 originated from two different origins and were reassorted in Yeonggwang, which is the intermediate point of two geographically separated groups.

中文翻译：

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在韩国番茄绿化病毒种群的系统发生特征：来自不同来源的分离株之间重新分类的证据。

番茄萎黄病毒（ToCV）是粉虱传播的韧皮部限制的crinivirus。2013年，在韩国的温室中观察到番茄叶片严重的静脉萎黄和青铜烫伤，这是已知的ToCV感染症状。为了鉴定有症状番茄植株中的ToCV感染，对2013年和2014年观察到ToCV样症状的11个番茄栽培区收集的叶片样品进行了ToCV特异性引物的RT-PCR。确认了大约一半的样品（45.18％）被ToCV感染，并鉴定出10种不同菌株的完整基因组。这是ToCV在韩国的首次报道。还分析了来自韩国和其他国家的ToCV分离株之间的亲缘关系和遗传变异。分别分析RNA1和RNA2时，ToCV分离株在系统树中分为三类，和ToCV韩国分离株被确认属于两个群体，在地理上是分开的。这些结果表明，韩国ToCV分离株起源于两个独立的起源。然而，证实了Yonggwang分离株的RNA1和RNA2序列属于不同的组，这表明ToCV RNA1和RNA2起源于两个不同的来源，并在Yonggwang进行了重配，Yonggwang是两个地理上分离的组的中间点。