Baby food has never been the object of biogenic amine profiling. The aim of this study was to develop a highly sensitive method for analysis of biogenic amines in ready-to-eat baby foods. The principle of the developed method involves high-performance liquid chromatography coupled to single-quadrupole mass spectrometry (HPLC–APCI–MS) of dansyl derivatives, presented also in comparison with common diode array and fluorescence detection systems. The confirmation of correct identification of derivatives was performed by in-source fragmentation of the product ion at 170 m/z, performed only in one MS analyzer. The method was used to identify the amine profile and quantify the putrescine, cadaverine, histamine, tyramine, spermidine, and spermine content in 68 ready-to-eat baby foods. The limits of detection and quantification were in the range of 0.07–1.67 and 0.2–5.0 ng mL− 1. The method enabled quantification of amines at ng/g level in almost all analyzed samples, without any preconcentration step. Amine recoveries of 86.0–105.2% were obtained with RSD ≤ 9.7%. The developed method could be used for quantification of the most frequently occurring BAs in foods including vegetables, fish, meat, or fruit at previously undetectable concentration levels, making the method multimatrix applicable and highly-sensitive.

中文翻译：

---

液相色谱-单四极杆质谱作为测定即食婴儿食品中生物胺的响应工具

婴儿食品从来都不是生物胺分析的对象。本研究的目的是开发一种高灵敏度的方法来分析即食婴儿食品中的生物胺。所开发方法的原理涉及与丹酰衍生物的单四极杆质谱 (HPLC-APCI-MS) 耦合的高效液相色谱法，还与常见的二极管阵列和荧光检测系统进行了比较。通过在 170 m/z 处对产物离子进行源内裂解来确认衍生物的正确鉴定，仅在一台 MS 分析仪中进行。该方法用于鉴定胺谱并量化 68 种即食婴儿食品中的腐胺、尸胺、组胺、酪胺、亚精胺和精胺的含量。检测限和定量限在 0.07–1 范围内。67 和 0.2–5.0 ng mL− 1。该方法能够对几乎所有分析样品中的 ng/g 水平的胺进行定量，无需任何预浓缩步骤。胺回收率为 86.0–105.2%，RSD ≤ 9.7%。开发的方法可用于定量分析食品中最常见的 BA，包括蔬菜、鱼、肉或水果，其浓度水平以前无法检测到，从而使该方法多矩阵适用且高度灵敏。