In the diagnostic pathology practice, specimens for electron microscopy (EM) are not necessarily handled and fixed under the ideal condition. In the present article, the author describes ultrastructural study using formalin-fixed and/or paraffin-embedded material. The fine morphologic preservation is often acceptable, particularly when small cubes are dug out of paraffin blocks. Particulate structures such as neuroendocrine granules and microbes are consistently observed even using paraffin sections. Paraffin sections signalized with silver particles in Grimelius and Grocott stains or diaminobenzidine products in immunostaining and in situ hybridization are applicable to EM evaluation by using a pre-embedding sequence. The practical merit includes the targeted approach: highly accurate sampling from focal lesions can be achieved for EM analysis, after observing hematoxylin and eosin-stained or specific-signalized paraffin-sections. This allows us pathologists a convenient and practical way for identifying focally infected pathogens, as well as a retrospective ultrastructural analysis of rare lesions long kept as a form of paraffin blocks.

中文翻译：

---

使用福尔马林固定的石蜡包埋材料进行的电子显微镜研究，特别是对微生物和内分泌颗粒的观察。

在诊断病理学实践中，不一定要在理想条件下处理和固定用于电子显微镜（EM）的标本。在本文中，作者描述了使用福尔马林固定和/或石蜡包埋的材料进行的超微结构研究。精细的形态保存通常是可以接受的，特别是当从石蜡块中挖出小方块时。即使使用石蜡切片，也能始终观察到颗粒状结构，例如神经内分泌颗粒和微生物。在免疫染色和原位杂交中，用Grimelius和Grocott染色中的银颗粒或二氨基联苯胺产物信号表示的石蜡切片可通过使用预嵌入序列进行EM评估。实际的优点包括有针对性的方法：可以从局灶性病变中进行高精度采样以进行EM分析，观察苏木精和曙红染色或特定信号的石蜡切片后。这为我们的病理学家提供了一种方便实用的方法，可用于识别局灶性感染的病原体，以及对长期保存为石蜡块形式的罕见病灶进行回顾性超微结构分析。