ABSTRACT Glioblastoma multiforme (GBM) is a malignant brain cancer that causes high mortality in patients. GBM responds weakly to the common cancer treatments such as chemotherapy and radiotherapy and even surgery. Carboplatin is an alkylating agent widely used to treat cancer. However, resistance to this drug is a common problem in its use in cancer treatment. Concomitant exposure to extremely low-frequency electromagnetic fields (ELF-EMFs) and carboplatin is one unexplored possibility for overcoming this resistance. Indeed, many lines of evidence show that EMF affects cancer cells and drug action. In this study, we evaluated the effect of concomitant administration of carboplatin and EMF (50 Hz, 70 G) and also concomitant administration of carboplatin and static magnetic field (SMF) (70 G) on human glioma cell line (U-87). The results showed that cotreatment reduced the efficiency of carboplatin in U-87 cells, by decreasing caspase-3 in comparison to drug groups. Overall, EMF reduced the apoptotic effect of carboplatin, possibly through a redox regulation mechanism. Therefore, we have to avoid coadministration of magnetic field (MF) and carboplatin in tumor area, because the MF decreased the toxicity of the drug. However, further studies are needed to reveal the action mechanism of this combination therapeutic method.

中文翻译：

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卡铂对人胶质母细胞瘤细胞的细胞毒性因同时暴露于极低频电磁场 (50 Hz, 70 G) 而降低

摘要 多形性胶质母细胞瘤（GBM）是一种恶性脑癌，患者死亡率高。GBM对化疗、放疗甚至手术等常见癌症治疗反应较弱。卡铂是一种广泛用于治疗癌症的烷化剂。然而，对这种药物的耐药性是其在癌症治疗中使用的一个常见问题。同时暴露于极低频电磁场 (ELF-EMF) 和卡铂是克服这种阻力的一种尚未探索的可能性。事实上，许多证据表明 EMF 会影响癌细胞和药物作用。在这项研究中，我们评估了卡铂和 EMF（50 Hz，70 G）同时给药以及卡铂和静磁场（SMF）（70 G）同时给药对人神经胶质瘤细胞系（U-87）的影响。结果表明，与药物组相比，共处理通过降低 caspase-3 降低了卡铂在 U-87 细胞中的效率。总体而言，EMF 可能通过氧化还原调节机制降低了卡铂的凋亡作用。因此，我们必须避免在肿瘤区域同时使用磁场（MF）和卡铂，因为MF降低了药物的毒性。然而，需要进一步的研究来揭示这种联合治疗方法的作用机制。因为MF降低了药物的毒性。然而，需要进一步的研究来揭示这种联合治疗方法的作用机制。因为MF降低了药物的毒性。然而，需要进一步的研究来揭示这种联合治疗方法的作用机制。