Objectives: Physical exercise significantly impacts the biochemistry of the organism. Ubiquinone is a key component of the mitochondrial respiratory chain and ubiquinol, its reduced and active form, is an emerging molecule in sport nutrition. The aim of this study was to evaluate the effect of ubiquinol supplementation on biochemical and oxidative stress indexes after an intense bout of exercise.

Methods: 21 male young athletes (26 + 5 years of age) were randomized in two groups according to a double blind cross-over study, either supplemented with ubiquinol (200 mg/day) or placebo for 1 month. Blood was withdrawn before and after a single bout of intense exercise (40 min run at 85% maxHR). Physical performance, hematochemical parameters, ubiquinone/ubiquinol plasma content, intracellular reactive oxygen species (ROS) level, mitochondrial membrane depolarization, paraoxonase activity and oxidative DNA damage were analyzed.

Results: A single bout of intense exercise produced a significant increase in most hematochemical indexes, in particular CK and Mb while, on the contrary, normalized coenzyme Q₁₀ plasma content decreased significantly in all subjects. Ubiquinol supplementation prevented exercise-induced CoQ deprivation and decrease in paraoxonase activity. Moreover at a cellular level, in peripheral blood mononuclear cells, ubiquinol supplementation was associated with a significant decrease in cytosolic ROS while mitochondrial membrane potential and oxidative DNA damage remained unchanged.

Discussion: Data highlights a very rapid dynamic of CoQ depletion following intense exercise underlying an increased demand by the organism. Ubiquinol supplementation minimized exercise-induced depletion and enhanced plasma and cellular antioxidant levels but it was not able to improve physical performance indexes or markers of muscular damage.

目标：体育锻炼显着影响生物的生物化学。泛醌是线粒体呼吸链的关键组成部分，泛醌，其还原和活性形式，是运动营养中的新兴分子。这项研究的目的是评估剧烈运动后补充泛醇对生化和氧化应激指标的影响。

方法：根据一项双盲交叉研究，将21名男性年轻运动员（26岁+ 5岁）随机分为两组，补充泛醇（200 mg /天）或安慰剂1个月。一次剧烈运动之前和之后抽取血液（以85％maxHR运行40分钟）。分析了物理性能，血液化学参数，泛醌/泛醇血浆含量，细胞内活性氧（ROS）水平，线粒体膜去极化，对氧磷酶活性和氧化性DNA损伤。

结果：一次剧烈运动后，大多数血液化学指标（尤其是CK和Mb）显着增加，而所有受试者中正常的辅酶Q ₁₀血浆含量均显着下降。补充泛醇可防止运动引起的辅酶Q剥夺和对氧磷酶活性的降低。此外，在细胞水平上，在外周血单核细胞中，补充泛醇与细胞质ROS的显着降低有关，而线粒体膜电位和氧化性DNA损伤保持不变。

讨论：数据表明，剧烈运动导致有机物需求增加后，辅酶Q消耗非常迅速。泛醇补充剂可最大程度地减少运动引起的消耗，并增强血浆和细胞的抗氧化剂水平，但不能改善身体机能指标或肌肉损伤的标志物。