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Dynamics of basal lamina fenestrations in the rat intestinal villous epithelium in response to dietary conditions.
Biomedical Research ( IF 1.2 ) Pub Date : 2018-04-20 , DOI: 10.2220/biomedres.39.65
Rie Azumi 1, 2 , Keisuke Morita 1, 2 , Yusuke Mizutani 2 , Manabu Hayatsu 2 , Shuji Terai 1 , Tatsuo Ushiki 2
Affiliation  

The epithelial basal lamina of the small intestine forms a felt-like sheet at the base of the epithelium. Previous studies have shown that the basal lamina has numerous fenestrations, which are produced by leukocytes penetrating through the basal lamina. In this study, we aimed to directly visualize fenestrations of the rat basal lamina in intestinal villi by scanning electron microscopy (SEM) after removal of the villous epithelium by osmium maceration and ultrasonic treatment. Structural changes in fenestrations were then investigated in relation to dietary conditions. SEM of these tissues revealed the presence of fenestrations in the villous epithelial basal lamina in all segments of the small intestine, although the number was the highest in the jejunum. The present study also showed that the number and size of fenestrations increased after feeding in the jejunum, whereas changes were unclear in the ileum. These findings suggested that the basal lamina fenestrations were changed through the dynamics of migrating leukocytes in dietary conditions and may also be related to the regulation of nutrient absorption, particularly as lipids are transported from the intercellular space of the epithelium to the lamina propria.

中文翻译:

饮食条件对大鼠肠道绒毛上皮基底层窗孔的动态影响。

小肠的上皮基底层在上皮底部形成毡状片。先前的研究表明,基底层有许多开孔,这些开孔是由穿透基底层的白细胞产生的。在这项研究中,我们旨在通过scanning浸和超声处理去除绒毛上皮后,通过扫描电子显微镜(SEM)直接观察小肠绒毛中大鼠基底层的开窗。然后研究开窗结构与饮食条件的关系。这些组织的SEM显示小肠所有部分的绒毛状上皮基底层中都存在开窗,尽管该数目在空肠中最高。本研究还表明,空腹进食后开窗的数量和大小增加,而回肠中的变化尚不清楚。这些发现表明,通过饮食条件下白细胞迁移的动力学改变了基底层开窗,并且还可能与营养吸收的调节有关,尤其是当脂质从上皮的细胞间空间转移至固有层时。
更新日期:2019-11-01
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