Algae are one of the natural materials used to green synthesis of nanoparticles. This method leads to minimize the toxicity of the chemical materials used to nanoparticle synthesis. In this study, zinc oxide nanoparticles (ZnO NPs) synthesized by Sargassum muticum algae extraction used to evaluate its cytotoxicity and apoptotic properties on human liver cancer cell line (HepG2). Trypan blue assay results demonstrate a concentration-dependent decrease in cell viability and MTT assay shows increased growth inhibition in time and dose-dependent manner. In addition, CAM assay confirmed the ability of ZnO NPs to inhibit angiogenesis, but chick morphology (both the CR and weight) was not changed. Apoptotic tests (annexin V/PI and AO/PI) show that green-synthesized ZnO NPs induce apoptosis in all three time points (24, 48 and 72h). Our results confirm the beneficial cytotoxic effects of green-synthesized ZnO NPs on Human liver cancer cell. This nanoparticle decreased angiogenesis and induces apoptosis, so we conclude that these nanoparticles can be used as a supplemental drug in cancer treatments.

中文翻译：

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使用马尾藻提取的绿色合成氧化锌纳米颗粒的抗血管生成和抗凋亡作用。

藻类是用于绿色合成纳米颗粒的天然材料之一。这种方法导致用于纳米粒子合成的化学材料的毒性最小化。在这项研究中，通过马尾藻提取物合成的氧化锌纳米颗粒（ZnO NPs）用于评估其对人肝癌细胞系（HepG2）的细胞毒性和凋亡特性。台盼蓝测定结果表明细胞活力呈浓度依赖性降低，而 MTT 测定显示以时间和剂量依赖性方式增加的生长抑制。此外，CAM 测定证实了 ZnO NPs 抑制血管生成的能力，但雏鸡形态（CR 和体重）没有改变。细胞凋亡测试（膜联蛋白 V/PI 和 AO/PI）显示绿色合成的 ZnO NPs 在所有三个时间点（24、48 和 72 小时）均诱导细胞凋亡。我们的结果证实了绿色合成的 ZnO NPs 对人肝癌细胞的有益细胞毒作用。这种纳米颗粒减少了血管生成并诱导细胞凋亡，因此我们得出结论，这些纳米颗粒可以用作癌症治疗的补充药物。