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Expression of Draxin in Lung Carcinomas.
Acta Histochemica et Cytochemica ( IF 2.4 ) Pub Date : 2018-04-07 , DOI: 10.1267/ahc.17035
Younosuke Sato 1 , Akira Matsuo 1 , Shinji Kudoh 1 , Liu Fang 1, 2 , Koki Hasegawa 3 , Yohei Shinmyo 4 , Takaaki Ito 1
Affiliation  

Guidance molecules, such as Netrin-1, and their receptors have important roles in controlling axon pathfinding, modulate biological activities of various cancer cells, and may be a useful target for cancer therapy. Dorsal repulsive axon guidance protein (Draxin) is a novel guidance molecule that binds not only common guidance molecule receptors with Netrin-1, but also directly binds the EGF domain of Netrin-1 through a 22-amino-acid peptide (22aa). By immunostaining, Draxin was positively expressed in small cell carcinoma, adenocarcinoma (ADC), and squamous cell carcinoma of the lung. In addition, western blot analysis revealed that Draxin was expressed in all histological types of lung cancer cell lines examined. Knockdown of Draxin in an ADC cell line H358 resulted in altered expression of molecules associated with proliferation and apoptosis. The Ki-67 labeling index of Draxin-knockdown ADC cells was increased compared to that of control ADC cells. In H358 cells, treatment of 22aa induced phosphorylation of histone H3, but did not change apoptosis-associated enzymes. These data suggest that Draxin might be involved in cell proliferation and apoptosis in lung adenocarcinoma cells.

中文翻译:

Draxin在肺癌中的表达。

诸如Netrin-1的指导分子及其受体在控制轴突寻路,调节各种癌细胞的生物学活性方面具有重要作用,并且可能是癌症治疗的有用靶标。背侧轴突导向蛋白(Draxin)是一种新型的导向分子,它不仅与Netrin-1结合常见的导向分子受体,而且还通过22个氨基酸的肽(22aa)直接结合Netrin-1的EGF域。通过免疫染色,Draxin在小细胞癌,腺癌(ADC)和肺鳞状细胞癌中阳性表达。此外,蛋白质印迹分析表明,Draxin在所有组织学类型的肺癌细胞系中均有表达。在ADC细胞系H358中抑制Draxin导致与增殖和凋亡相关的分子表达改变。与对照ADC细胞相比,Draxin-knockdown ADC细胞的Ki-67标记指数增加。在H358细胞中,22aa的处理诱导了组蛋白H3的磷酸化,但没有改变细胞凋亡相关的酶。这些数据表明Draxin可能参与了肺腺癌细胞的细胞增殖和凋亡。
更新日期:2019-11-01
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