Identification of fusion genes in cancer is essential for pathological diagnosis and clinical therapy. Although methods for detection of fusion genes, such as fluorescence in situ hybridization (FISH) and real-time polymerase chain reaction (PCR), have been developed in last two decades, these methods are not ideal for detection of these genetic alterations owing to their high cost and time-consuming procedures. In this study, we developed novel application for detection of gene translocations using loop-mediated isothermal amplification (LAMP). We verified the amplified DNA products of echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase (EML4-ALK), synaptotagmin and synovial sarcoma, X breakpoint (SYT-SSX), and immunoglobulin heavy chain gene and B cell leukemia/lymphoma 2 (IgH/BCL2) by real-time PCR, agarose-gel electrophoresis, and the naked eye after the LAMP procedure. Fusion genes were detected in samples diluted 103 times within 60 min. Because of the advantages of rapid amplification, simple operation, and easy detection without requiring sophisticated equipment or technical skill, LAMP may have potential applications as an on-site analytical approach in hospitals for pathological diagnosis and decision making regarding appropriate therapeutic approachs.

中文翻译：

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循环介导的等温扩增在基因转运快速检测中的新应用。

癌症中融合基因的鉴定对于病理诊断和临床治疗至关重要。尽管过去二十年来已经开发出了检测融合基因的方法，例如荧光原位杂交（FISH）和实时聚合酶链反应（PCR），但是由于这些方法的检测，这些方法并不是理想的检测方法高成本和费时的程序。在这项研究中，我们开发了使用环介导的等温扩增（LAMP）检测基因易位的新应用。我们验证了棘皮动物微管相关蛋白样4和间变性淋巴瘤激酶（EML4-ALK），突触结合蛋白和滑膜肉瘤，X断点（SYT-SSX）和免疫球蛋白重链基因和B细胞白血病/淋巴瘤2的扩增DNA产物。 （IgH / BCL2）通过实时PCR进行，琼脂糖凝胶电泳，LAMP程序后肉眼观察。在60分钟内稀释103倍的样品中检测到融合基因。由于快速扩增，简单的操作和易于检测的优点而无需复杂的设备或技术技能，LAMP可能作为医院中的现场分析方法具有潜在的应用价值，可用于病理诊断和有关适当治疗方法的决策。