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Comparison of RAFT derived Poly(vinylpyrolidone) verses Poly(oligoethyleneglycol methacrylate) for the Stabilization of Glycosylated Gold Nanoparticles.
Journal of Polymer Science ( IF 3.4 ) Pub Date : 2017-01-26 , DOI: 10.1002/pola.28481
Nga Sze Ieong 1 , Caroline I Biggs 1 , Mark Walker 1, 2 , Matthew I Gibson 1, 3
Affiliation  

Carbohydrates dictate many biological processes including infection by pathogens. Glycosylated polymers and nanomaterials which have increased affinity due to the cluster glycoside effect, are therefore useful tools to probe function, but also as prophylactic therapies or diagnostic tools. Here, the effect of polymer structure on the coating of gold nanoparticles is studied in the context of grafting density, buffer stability and in a lectin binding assay. RAFT polymerization is used to generate poly(oligoethyleneglycol methacrylates) and poly(N-vinyl pyrolidones) with a thiol end-group for subsequent immobilization onto the gold. It is observed that poly(oligoethylene glycol methacrylates), despite being widely used particle coatings, lead to low grafting densities which in turn resulted in lower stability in biological buffers. A depression of the cloud point upon nanoparticle immobilization is also seen, which might compromise performance. In comparison poly(vinyl pyrolidones) resulted in stable particles with higher grafting densities due to the compact size of each monomer unit. The higher grafting density also enabled an increase in the number of carbohydrates which can be installed per nanoparticle at the chain ends, and gave increased binding in a lectin recognition assay. These results will guide the development of new nanoparticle biosensors with enhanced specificity, affinity and stability.

中文翻译:

RAFT 衍生的聚(乙烯基吡咯烷酮)与聚(低聚乙二醇甲基丙烯酸酯)对糖基化金纳米颗粒的稳定作用的比较。

碳水化合物决定许多生物过程,包括病原体感染。因此,糖基化聚合物和纳米材料由于簇糖苷效应而具有增加的亲和力,因此是探测功能的有用工具,而且也可作为预防性治疗或诊断工具。在此,在接枝密度、缓冲液稳定性和凝集素结合测定的背景下研究了聚合物结构对金纳米颗粒涂层的影响。RAFT 聚合用于生成带有硫醇端基的聚(低聚乙二醇甲基丙烯酸酯)和聚(N-乙烯基吡咯烷酮),用于随后固定到金上。据观察,聚(低聚乙二醇甲基丙烯酸酯)尽管被广泛使用的颗粒涂层,但其接枝密度较低,进而导致生物缓冲液的稳定性较低。还可以看到纳米颗粒固定后浊点降低,这可能会损害性能。相比之下,由于每个单体单元的尺寸紧凑,聚(乙烯基吡咯烷酮)产生具有更高接枝密度的稳定颗粒。较高的接枝密度还能够增加每个纳米颗粒链末端可安装的碳水化合物的数量,并在凝集素识别测定中增加结合力。这些结果将指导开发具有增强的特异性、亲和力和稳定性的新型纳米粒子生物传感器。
更新日期:2019-11-01
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