An electrochemical aptasensor for detection of trace amounts of aflatoxin M1 was developed. This required immobilization of aptamer on screen printed gold electrode comprising of working electrode, counter electrode and reference electrode and was achieved by sequentially layering dithiodipropionic acid, streptavidin and biotinylated-tetraethylene glycol-aptamer. Immobilization of aptamer was monitored by cyclic voltammetry. Peak current in square wave voltammogram was inversely related to logarithmic concentration of aflatoxin M1. Dynamic range of sensor was 1–10⁵ ppt aflatoxin M1. Sensor can be regenerated by treating electrode with 10% sodium dodecyl sulfate or 40 mM tris-HCl (pH 8.0) containing 10 mM ethylenediaminetetraacetic acid and 0.02% tween-20.

中文翻译：

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固定在金电极上的适体可检测痕量的黄曲霉毒素M1。

开发了一种用于检测痕量黄曲霉毒素M1的电化学适体传感器。这需要将适体固定在由工作电极，对电极和参比电极组成的丝网印刷金电极上，并且是通过依次层叠二硫代二丙酸，链霉亲和素和生物素化的四甘醇适体来实现的。通过循环伏安法监测适体的固定。方波伏安图中的峰值电流与黄曲霉毒素M1的对数浓度成反比。传感器的动态范围是1–10 ⁵  ppt黄曲霉毒素M1。可以通过用10％的十二烷基硫酸钠或40 mM的tris-HCl（pH 8.0）（含10 mM乙二胺四乙酸和0.02％tween-20）处理电极来再生传感器。