Persistent accumulation of immune cells mediated by α4β1 integrin (VLA-4) is a hallmark of the inflammatory diseases and of chronic inflammation observed in the affected tissues of autoimmune diseases. Aiming at exploring new methods for monitoring the course of the inflammatory processes, we designed the first peptide-functionalized nanostructured devices capable to mimic the high-density multivalency binding between the α4β1 integrin-expressing cells and the ligands overexpressed on the endothelial surfaces, in the proximity of the sites of inflammation. Specifically, we describe the first examples of monolayers constituted by dye-loaded zeolite L crystals, coated with α4β1 integrin peptide ligands, and we analyze the adhesion of model Jurkat cells in comparison to non-α4β1 integrin-expressing cells. In particular, the peptidomimetic diphenylurea-Leu-Asp-Val-diamine allows significant and selective detection of α4β1 integrin-expressing Jurkat cells, after very rapid incubation time, supporting the possible implementation in a diagnostic device capable to detect the desired cells from biological fluids, obtainable from patients in a noninvasive way.

中文翻译：

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使用肽功能化的纳米结构材料模拟与炎症部位相邻的内皮表面，选择性检测表达α4β1整联蛋白（VLA-4）的细胞。

由α4β1整联蛋白（VLA-4）介导的免疫细胞的持久积累是炎症性疾病和自身免疫性疾病受影响组织中观察到的慢性炎症的标志。为了探索监测炎症过程的新方法，我们设计了首个肽官能化的纳米结构装置，该装置能够模拟表达α4β1整合素的细胞与内皮表面过表达的配体之间的高密度多价结合。炎症部位附近。具体而言，我们描述了由载有α4β1整联蛋白肽配体的染料加载的沸石L晶体构成的单层的第一个例子，并且与非α4β1整联蛋白表达细胞相比，我们分析了Jurkat模型细胞的粘附性。特别是，