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Application of DNA markers for discrimination between Japanese and Australian Wagyu beef.
Animal Science Journal ( IF 2 ) Pub Date : 2017-10-31 , DOI: 10.1111/asj.12938
Fuki Kawaguchi 1 , Yuto Kitamura 1 , Ryuji Nakajima 2 , Masayoshi Takahashi 2 , Hiroaki Goto 2 , Yuichi Washida 3 , Yoshikazu Yamamoto 4 , Shinji Sasazaki 1 , Hideyuki Mannen 1
Affiliation  

The objective of this study was to discriminate between original Japanese and Australian Wagyu beef, which is sold in the Singapore markets, using six previously developed DNA markers. To effectively evaluate the six markers for breed identification, the probability of identification as Australian Wagyu beef was calculated based on the estimated allele frequencies using 130 Australian Wagyu individuals. The combined use of six markers would allow the discrimination of Australian Wagyu beef with an estimated probability of 0.776. The probability to discriminate Australian Wagyu from Japanese Wagyu beef was sufficiently high. In addition, Australian Wagyu has maternal mitochondrial DNA of Bos indicus cattle with moderate high frequency of 0.377. The DNA marker system could also be used as a deterrent force against false sales, and contribute to the reduction and prevention of incorrect or falsified labeling of beef.

中文翻译:

DNA标记在区分日本和澳大利亚和牛牛肉中的应用。

这项研究的目的是使用六个以前开发的DNA标记物来区分在新加坡市场上出售的日本和澳大利亚原和牛。为了有效评估用于品种识别的六个标记,基于估计的等位基因频率,使用130位澳大利亚和牛个体,计算出被鉴定为澳大利亚和牛牛肉的概率。结合使用六个标记可以估计澳大利亚和牛牛肉,估计概率为0.776。区分澳大利亚和牛与日本和牛的可能性很高。此外,澳大利亚和牛的印度产Bos indicus牛的线粒体DNA中等频率为0.377。DNA标记系统还可以用作制止虚假销售的威慑力量,
更新日期:2019-11-01
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