The submandibular gland (SMG) is one of the major salivary glands that play important roles for variety of physiological functions, such as digestion of foods, prevention of infection, and lubrication of the mouth. Dysfunction of the SMG, often associated with a salivary inflammation, adversely influences a person's quality of life. However, the mechanism underlying inflammation-driven dysfunction of the SMG is largely unknown. Here, we used a mouse model in which the main excretory duct of the SMG is ligated unilaterally to induce inflammation of the gland and examined the expression of Wnt5a, Ror1 and Ror2 genes, encoding Wnt5a ligand and its cognate receptors, which have been implicated in tissue damage or inflammatory responses in variety of tissues. We show that expression levels of Ror1, Ror2, and Wnt5a are increased in the ligated SMG undergoing interstitial fibrosis, which is accompanied by robust expression of fibrosis-associated genes, such as TGF-β1, TNF-α, IL-1β, and MMP-2. Increased immunostaining signal of Ror2 was detected in the fibrotic tissues with abundant accumulation of fibroblasts and collagen fibers in the ligated SMG, suggesting that Ror2-mediated signaling might be activated in response to tissue damage and associated with progression of fibrosis in the SMG.Key words: submandibular gland, Ror2, Wnt5a, fibrosis, inflammation.

中文翻译：

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Ror2的表达与下颌下腺纤维化相关。

颌下腺（SMG）是主要的唾液腺之一，对多种生理功能（例如食物的消化，感染的预防和口腔的润滑）起着重要的作用。SMG的功能障碍通常与唾液发炎有关，会对人的生活质量产生不利影响。然而，SMG炎症驱动功能障碍的潜在机制尚不清楚。在这里，我们使用的小鼠模型中，单侧结扎了SMG的主要排泄管以诱发腺体炎症，并检查了编码Wnt5a配体及其同源受体的Wnt5a，Ror1和Ror2基因的表达。组织损伤或各种组织的炎症反应。我们显示Ror1，Ror2，在进行间质性纤维化的连接的SMG中，Wnt5a和Wnt5a的表达增加，同时伴随着与纤维化相关的基因如TGF-β1，TNF-α，IL-1β和MMP-2的强表达。在结扎的SMG中，在纤维化组织中检测到Ror2的免疫染色信号增加，其中成纤维细胞和胶原纤维大量积累，这表明Ror2介导的信号转导可能响应于组织损伤并与SMG中纤维化的进展相关。 ：下颌下腺，Ror2，Wnt5a，纤维化，炎症。