We determined the effects of atmospheric temperature (10-30 ± 2°C in 5°C increments) and carbon dioxide (CO₂) levels (400 ± 50 ppm, 540 ± 50 ppm, and 940 ± 50 ppm) on the infection of Solanum tuberosum cv. Chubaek by Potato leafroll virus (PLRV). Below CO₂ levels of 400 ± 50 ppm, the PLRV infection rate and RNA content in plant tissues increased as the temperature increased to 20 ± 2°C, but declined at higher temperatures. At high CO₂ levels (940 ± 50 ppm), more plants were infected by PLRV at 30 ± 2°C than at 20 or 25 ± 2°C, whereas PLRV RNA content was unchanged in the 20-30 ± 2°C temperature range. The effects of atmospheric CO₂ concentration on the acquisition of PLRV by Myzus persicae and accumulation of PLRV RNA in plant tissues were investigated using a growth chamber at 20 ± 2°C. The M. persicae PLRV RNA content slightly increased at elevated CO₂ levels (940 ± 50 ppm), but this increase was not statistically significant. Transmission rates of PLRV by Physalis floridana increased as CO₂ concentration increased. More PLRV RNA accumulated in potato plants maintained at 540 or 940 ± 50 ppm CO₂, than in plants maintained at 400 ± 50 ppm. This is the first evidence of greater PLRV RNA accumulation and larger numbers of S. tuberosum plants infected by PLRV under conditions of combined high CO₂ levels (940 ± 50 ppm) and high temperature (30 ± 2°C).

中文翻译：

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温度和CO2水平影响马铃薯马铃薯卷叶病毒感染。

我们确定了大气温度（10-30±2°C，以5°C为增量）和二氧化碳（CO ₂）水平（400±50 ppm，540±50 ppm和940±50 ppm）的影响马铃薯品种。马铃薯卷叶病毒（PLRV）产生的Chubaek 。在CO ₂浓度低于400±50 ppm时，温度升高至20±2°C时，植物组织中的PLRV感染率和RNA含量增加，但在较高温度下则下降。在高CO ₂水平（940±50 ppm）下，在30±2°C下感染PLRV的植物比在20或25±2°C下感染的植物多，而PLRV RNA含量在20-30±2°C的温度下保持不变范围。大气中CO ₂浓度对PLRV获取的影响使用生长室在20±2°C下研究桃蚜和植物组织中PLRV RNA的积累。的桃蚜PLRV RNA含量在升高的CO略有增加₂水平（940±50ppm的），但这种增加不是统计学显著。酸浆对PLRV的传播速率随CO ₂浓度的增加而增加。与维持在400±50 ppm的植物相比，保持在540或940±50 ppm CO _2的马铃薯植物中积累的PLRV RNA更多。这是在合并的高CO ₂条件下PLRV RNA积累量更大和被PLRV感染的马铃薯的第一个证据。 水平（940±50 ppm）和高温（30±2°C）。