The complete genome sequence of a Slovak SL-1 isolate of Tomato mosaic virus (ToMV) was determined from the next generation sequencing (NGS) data, further confirming a limited sequence divergence in this tobamovirus species. Tomato genotypes Monalbo, Mobaci and Moperou, respectively carrying the susceptible tm-2 allele or the Tm-1 and Tm-2 resistant alleles, were tested for their susceptibility to ToMV SL-1. Although the three tomato genotypes accumulated ToMV SL-1 to similar amounts as judged by semi-quantitative DAS-ELISA, they showed variations in the rate of infection and symptomatology. Possible differences in the intra-isolate variability and polymorphism between viral populations propagating in these tomato genotypes were evaluated by analysis of the capsid protein (CP) encoding region. Irrespective of genotype infected, the intra-isolate haplotype structure showed the presence of the same highly dominant CP sequence and the low level of population diversity (0.08-0.19%). Our results suggest that ToMV CP encoding sequence is relatively stable in the viral population during its replication in vivo and provides further demonstration that RNA viruses may show high sequence stability, probably as a result of purifying selection.

中文翻译：

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在衣壳蛋白编码区导致低病毒种群异质性的番茄花叶病毒对番茄不同基因型的实验感染。

斯洛伐克花叶病毒SL-1分离株的完整基因组序列（ToMV）是由下一代测序（NGS）数据确定的，进一步证实了该烟草花叶病毒物种中有限的序列差异。测试了分别携带易感tm-2等位基因或Tm-1和Tm-2抗性等位基因的番茄基因型Monalbo，Mobaci和Moperou对ToMV SL-1的敏感性。尽管通过半定量DAS-ELISA判断，这三种番茄基因型积累的ToMV SL-1数量相似，但它们显示出感染率和症状的变化。通过衣壳蛋白（CP）编码区域的分析，评估了在这些番茄基因型中繁殖的病毒种群之间的分离内变异性和多态性的可能差异。不论感染了哪种基因型，分离物中的单倍型结构显示存在相同的高度显性的CP序列和较低的种群多样性水平（0.08-0.19％）。我们的结果表明，ToMV CP编码序列在体内复制过程中在病毒种群中相对稳定，并进一步证明RNA病毒可能显示出较高的序列稳定性，这可能是纯化选择的结果。