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Evaluation of platelet activation in leukocyte-depleted platelet concentrates during storage.
Biomolecules and Biomedicine ( IF 3.4 ) Pub Date : 2018-02-20 , DOI: 10.17305/bjbms.2017.2321
Miodrag Vucic 1 , Zoran Stanojkovic , Ana Antic , Jelana Vucic , Voja Pavlovic
Affiliation  

Structural and functional changes in platelets during storage can lead to the loss of platelet reactivity and response. Our aim was to evaluate leukocyte-depleted platelet concentrates on storage days 0, 3 and 5, obtained by in-line filtration. In non-filtered platelet concentrates (NF-PC) group, 180 whole blood units were collected with quadruple blood bags and then compared to another group of 180 whole blood units (leukocyte-depleted platelet concentrates [LD-PC]), collected in Imuflex Whole Blood Filter Saving Platelets (WB-SP) bags with an integrated leukoreduction filter, with regard to the platelet quality and characteristics. The efficacy of the two techniques for platelet concentrate preparation was evaluated by white blood cell (WBC) and platelet count on day 0. The partial pressure of oxygen (pO2), pH, platelets positive for P-selectin (CD62P), CD63, cluster of differentiation 42b (CD42b), phosphatidylserine (PS), and mitochondrial membrane potential (MMP) were analyzed during the storage in both groups. A significantly lower WBC count and higher platelet count was observed in LD-PC compared to NF-PC group, indicating the overall efficacy of the first technique. During the 5-day storage, pH and pO2 decreased in both groups. In LD-PC group, higher pH, increased pO2 and decreased platelet surface expression of CD62P, CD63 and PS were observed compared to NF-PC group. In both groups, the percentage of CD42b positive platelets and MMP did not change significantly during the 5-day period. The assessment of different markers of platelet activation may be an effective tool in evaluating the quality of platelets during storage. A better understanding of platelet activation may provide new insights for developing a novel therapeutic approach in the manipulation of platelet aggregation.

中文翻译:

储存过程中贫白细胞贫血小板浓缩物中血小板活化的评估。

储存过程中血小板的结构和功能变化会导致血小板反应性和反应能力下降。我们的目的是评估在0、3和5天通过在线过滤获得的贫白细胞血小板浓缩液。在未过滤的浓缩血小板(NF-PC)组中,用四重血袋收集180个全血单位,然后与在Imuflex中收集的另一组180个全血单位(贫白细胞血小板浓缩物[LD-PC])进行比较考虑到血小板的质量和特性,配有集成白细胞减少过滤器的全血过滤保存血小板(WB-SP)袋。在第0天通过白细胞(WBC)和血小板计数评估了这两种技术对血小板浓缩液的制备效果。氧分压(pO2),pH,两组在储存期间均分析了P-选择蛋白(CD62P),CD63,分化簇42b(CD42b),磷脂酰丝氨酸(PS)和线粒体膜电位(MMP)阳性的血小板。与NF-PC组相比,LD-PC的WBC计数显着降低,血小板计数更高,这表明第一种技术的整体疗效。在5天的储存期间,两组的pH和pO2均下降。与NF-PC组相比,LD-PC组可观察到较高的pH值,pO2增加和CD62P,CD63和PS的血小板表面表达降低。两组中,CD42b阳性血小板和MMP的百分比在5天期间均无明显变化。评估血小板活化的不同标志物可能是评估储存过程中血小板质量的有效工具。
更新日期:2020-08-21
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