Herpes simplex virus type 1 (HSV-1) causes significant human diseases ranging from skin lesions to encephalitis, especially in neonates and immunocompromised hosts. The discovery of novel anti-HSV-1 drugs with low toxicity is required for public health. Arbidol hydrochloride (ARB) is an indole derivative molecule with broad-spectrum antiviral activity. In this study, the antiviral effects of ARB against HSV-1 infection were evaluated in vitro and in vivo. The results showed that ARB presents significant inhibitory effect on HSV-1 plaque formation and generation of progeny virus, with EC50 values (50% effective concentration) of 5.39 µg/mL (10.49 µM) and 2.26 µg/mL (4.40 µM), respectively. Moreover, time-of-addition and time-of-removal assays further suggested that ARB has viral inhibitory effects when added up to 12 h post-infection (p.i.), which could be further corroborated by determining the expression of viral immediate-early (ICP4, ICP22 and ICP27), early (ICP8 and UL42) and late (gB, gD, gH, VP1/2 and VP16) genes by real-time quantitative PCR as well as the expression of viral protein ICP4 and ICP8 at 6 h and 12 h p.i. Results of the in vivo study showed that ARB could reduce guinea pig skin lesions caused by HSV-1 infection. Conclusively, this report offers new perspectives in the search for therapeutic measures in the treatment of HSV-1 infection.

中文翻译：

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盐酸阿比多尔在体外和体内对单纯性疱疹病毒I的抗病毒活性。

1型单纯疱疹病毒（HSV-1）引起严重的人类疾病，从皮肤损害到脑炎，尤其是在新生儿和免疫功能低下的宿主中。公共卫生需要发现低毒性的新型抗HSV-1药物。盐酸阿比多尔（ARB）是具有广谱抗病毒活性的吲哚衍生物分子。在这项研究中，在体外和体内评估了ARB对HSV-1感染的抗病毒作用。结果表明，ARB对HSV-1斑块形成和子代病毒的产生具有明显的抑制作用，EC50值（50％有效浓度）分别为5.39 µg / mL（10.49 µM）和2.26 µg / mL（4.40 µM）。 。此外，添加时间和去除时间分析进一步表明，在感染后（pi）最多添加12 h时，ARB具有病毒抑制作用，通过实时定量PCR测定病毒的早期（ICP4，ICP22和ICP27），早期（ICP8和UL42）和晚期（gB，gD，gH，VP1 / 2和VP16）基因的表达可以进一步证实以及在感染后6 h和12 h病毒蛋白ICP4和ICP8的表达体内研究结果表明ARB可以减轻HSV-1感染引起的豚鼠皮肤损伤。总之，本报告为寻找HSV-1感染的治疗方法提供了新的见解。体内研究结果表明，ARB可以减轻HSV-1感染引起的豚鼠皮肤损伤。总之，本报告为寻找HSV-1感染的治疗方法提供了新的见解。体内研究结果表明，ARB可以减轻HSV-1感染引起的豚鼠皮肤损伤。总之，本报告为寻找HSV-1感染的治疗方法提供了新的见解。