Bacterial separation from human blood samples can help with the identification of pathogenic bacteria for sepsis diagnosis. In this work, we report an acoustofluidic device for label-free bacterial separation from human blood samples. In particular, we exploit the acoustic radiation force generated from a tilted-angle standing surface acoustic wave (taSSAW) field to separate E. coli from human blood cells based on their size difference. Flow cytometry analysis of the E. coli separated from red blood cells (RBCs) shows a purity of more than 96%. Moreover, the label-free electrochemical detection of the separated E. coli displays reduced non-specific signals due to the removal of blood cells. Our acoustofluidic bacterial separation platform has advantages such as label-free separation, high biocompatibility, flexibility, low cost, miniaturization, automation, and ease of in-line integration. The platform can be incorporated with an on-chip sensor to realize a point-of-care (POC) sepsis diagnostic device.

中文翻译：

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声流体细菌分离

从人体血液样本中分离细菌有助于鉴定用于脓毒症诊断的病原菌。在这项工作中，我们报告了一种用于从人体血液样本中进行无标记细菌分离的声流体装置。特别是，我们利用倾斜角驻波表面声波 (taSSAW) 场产生的声辐射力，根据大小差异将大肠杆菌与人类血细胞分离。从红细胞 (RBC) 中分离的大肠杆菌的流式细胞术分析显示纯度超过 96%。此外，由于去除了血细胞，分离的大肠杆菌的无标记电化学检测显示减少的非特异性信号。我们的声流体细菌分离平台具有免标记分离、高生物相容性、灵活性、低成本、小型化、自动化和易于在线集成。该平台可以与片上传感器相结合，以实现即时 (POC) 败血症诊断设备。