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Antitumor Effect of a DNA Vaccine Harboring Prostate Cancer-Specific Antigen with IL-12 as an Intramolecular Adjuvant.
Microbial Physiology ( IF 1.2 ) Pub Date : 2017-08-05 , DOI: 10.1159/000477245
Yu Yang 1 , Zhiqiang Shao , Jiangping Gao
Affiliation  

To improve the lower immune intensity of DNA vaccines, we developed a DNA vaccine based on prostate cancer-specific antigen (PSA), which has been suggested as a potential target for prostate cancer therapy, and enhanced the DNA vaccine potency using interleukin-12 (IL-12) as an intramolecular adjuvant. A series of DNA plasmids encoding human PSA, IL-12, and their conjugates was constructed and injected into female mice intramuscularly, followed by an electric pulse. The humoral and cellular immune responses after immunization were detected by ELISA and ELISPOT, respectively. To evaluate the therapeutic efficacy of these plasmids, a mouse model with a PSA-expressing tumor was constructed. Mice vaccinated with PSA-IL-12 plasmids elicited the strongest PSA-specific humoral and cellular immune responses. Furthermore, these vaccinations inhibited the growth of PSA-expressing tumors and prolonged mouse survival. These observations emphasize the potential of the IL-12 gene as an intramolecular adjuvant for DNA vaccines. Moreover, the vaccine based on PSA and IL-12 may be a promising treatment for prostate cancer.

中文翻译:

具有IL-12作为分子内佐剂的带有前列腺癌特异性抗原的DNA疫苗的抗肿瘤作用。

为了提高DNA疫苗的较低免疫强度,我们开发了一种基于前列腺癌特异性抗原(PSA)的DNA疫苗,该疫苗已被建议作为前列腺癌治疗的潜在靶标,并使用白介素12( IL-12)作为分子内佐剂。构建了一系列编码人PSA,IL-12及其结合物的DNA质粒,并将其肌肉注射到雌性小鼠体内,然后进行电脉冲注射。分别通过ELISA和ELISPOT检测免疫后的体液和细胞免疫应答。为了评估这些质粒的治疗功效,构建了具有表达PSA的肿瘤的小鼠模型。接种了PSA-IL-12质粒的小鼠引发了最强的PSA特异性体液和细胞免疫应答。此外,这些疫苗抑制了表达PSA的肿瘤的生长并延长了小鼠的存活时间。这些观察结果强调了IL-12基因作为DNA疫苗分子内佐剂的潜力。此外,基于PSA和IL-12的疫苗可能是前列腺癌的有前途的治疗方法。
更新日期:2019-11-01
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