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Measurement of lipid transfer proteins in genetically engineered maize using liquid chromatography with tandem mass spectrometry (LC-MS/MS).
GM Crops & Food ( IF 3.9 ) Pub Date : 2017-08-02 , DOI: 10.1080/21645698.2017.1349602
Ryan C Hill 1 , Xiujuan Wang 1 , Barry W Schafer 1 , Satyalinga Srinivas Gampala 1 , Rod A Herman 1
Affiliation  

Endogenous allergenicity evaluation is a required part of the risk assessment for genetically engineered (GE) crops. Although maize is not considered a major allergenic food, a lipid transfer protein (Zea m 14) in maize grain has been identified as a potential IgE-mediated food allergen. Currently, the relationship between allergen exposure and risk of sensitization is not well understood. Hence, reliable quantitative methods are useful for determining the natural range and variability of allergen levels across multiple geographies and genetic backgrounds. A LC-MS/MS analytical method was developed and validated in our laboratory to quantify Zea m 14 in grain from 2 GE maize hybrids and 20 non-GE maize hybrids. The measured Zea m 14 levels in GE maize grain and conventional non-GE maize grain ranged from 146.87 to 574.93 ng/mg across 16 field sites located in the United States and Argentina. The method accurately quantified endogenous Zea m 14 from maize grain and results show Zea m 14 levels in the GE maize varieties were within the natural variation observed in traditionally bred non-GE maize.

中文翻译:

使用液相色谱-串联质谱(LC-MS / MS)测定基因工程玉米中的脂质转移蛋白。

内源性致敏性评估是基因工程(GE)作物风险评估的必要部分。尽管玉米不被认为是主要的过敏原食品,但玉米粒中的脂质转移蛋白(Zea m 14)已被确定为潜在的IgE介导的食物过敏原。目前,过敏原暴露与致敏风险之间的关系尚不清楚。因此,可靠的定量方法可用于确定跨多个地区和遗传背景的过敏原水平的自然范围和变异性。开发了一种LC-MS / MS分析方法,并在我们的实验室中进行了验证,以定量分析来自2个GE玉米杂交种和20个非GE玉米杂交种的谷物中的玉米14号玉米。在转基因玉米和常规非转基因玉米中测得的Zea m 14含量范围为146.87至574。位于美国和阿根廷的16个现场站点的浓度为93 ng / mg。该方法准确地定量了玉米籽粒中的内源性Zea m 14,结果表明,GE玉米品种中Zea m 14的水平在传统育种的非GE玉米中观察到的自然变异范围内。
更新日期:2019-11-01
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