The ciliary zonules link the lens to the ciliary body in the eye, controlling the thickness of the lens for focusing through their characteristic elasticity. The ciliary zonules are composed of oxytalan fibers. Physiological or pathological damage to the ciliary zonules, including exposure to ultraviolet (UV)-A and UV-B components, can lead to lens dislocation. However, no studies have shown whether UV affects the ciliary zonule. Here, we assessed the effects of UV light on human nonpigmented ciliary epithelial cells (HNPCECs). HNPCECs were cultured for 4 weeks, and expression of fibrillin-1 and fibrillin-2 was confirmed. In control cultures (0 mJ/cm2), some fibrillin-1-positive fibers were merged with fibrillin-2. After UV-A irradiation, the appearance of both fibrillin-1- and fibrillin-2-positive fibers was unchanged. However, after UV-B irradiation, fibrillin-1-positive fibers became thin at an irradiation level of 100 mJ/cm2, and the fiber structure became amorphous at 150 mJ/cm2. Fibrillin-2-positive fibers lost their continuity and disappeared after being exposed to 150 mJ/cm2 UV-B. UV-B irradiation did not affect cell viability, possibly because of the sensitivity of fibrillin-1 and fibrillin-2 to UV-B. Thus, dislocation of the lens with age may be attributable to cumulative exposure to UV-B.

中文翻译：

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紫外线B对人非色素性睫状上皮细胞中Fibrillin-1和Fibrillin-2的影响。

睫状小带将晶状体连接到眼睛的睫状体，通过其特有的弹性控制晶状体的厚度以聚焦。睫状小带由氧化钽纤维组成。睫状小带的生理或病理损伤（包括暴露于紫外线（UV）-A和UV-B成分）会导致晶状体脱位。但是，没有研究表明紫外线是否会影响睫状小带。在这里，我们评估了紫外线对人非色素性睫状上皮细胞（HNPCECs）的影响。将HNPCEC培养4周，并确认了原纤维蛋白-1和原纤维蛋白2的表达。在对照培养物中（0 mJ / cm2），一些原纤维蛋白-1阳性纤维与原纤维蛋白-2融合。在UV-A照射之后，原纤维蛋白-1和原纤维蛋白-2阳性纤维的外观均未改变。然而，在UV-B照射之后，原纤维蛋白-1阳性纤维在100mJ / cm 2的照射水平下变细，并且纤维结构在150mJ / cm 2的情况下变为非晶态。暴露于150 mJ / cm2 UV-B后，原纤维蛋白2阳性纤维失去连续性并消失。UV-B辐照不会影响细胞活力，可能是因为原纤维蛋白1和原纤维蛋白2对UV-B的敏感性。因此，随着年龄的增长，晶状体脱位可归因于累积暴露于UV-B。