Growth arrest specific 2 (gas2) gene is a component of the microfilament system that plays a major role in the cell cycle, regulation of microfilaments, and cell morphology during apoptotic processes. However, little information is available on fish gas2. In this study, the tilapia (Oreochromis niloticus) gas2 gene was cloned and characterized for the first time. The open reading frame was 1020 bp, encoding 340 amino acids; the 5′-untranslated region (UTR) was 140 bp and the 3′-UTR was 70 bp, with a poly (A) tail. The highest promoter activity occurred in the regulatory region (–3000 to –2400 bp). The Gas2-GFP fusion protein was distributed within the cytoplasm. Quantitative reverse transcription-polymerase chain reaction and western blot analyses revealed that gas2 gene expression levels in the liver, muscle, and brain were clearly affected by low temperature stress. The results of gas2 RNAi showed decreased expression of the gas2 and P53 genes. These results suggest that the tilapia gas2 gene may be involved in low temperature stress-induced apoptosis.

中文翻译：

---

罗非鱼（Oreochromis niloticus）中的生长停滞特定基因2：低温胁迫下的分子表征和功能分析。

生长停滞特异性2（gas2）基因是微丝系统的一个组成部分，在细胞周期，微丝调节和凋亡过程中的细胞形态中起主要作用。但是，关于鱼气的信息很少。在这项研究中，罗非鱼（Oreochromis niloticus）gas2基因被首次克隆和鉴定。开放阅读框为1020 bp，编码340个氨基酸。5'-非翻译区（UTR）为140 bp，3'-UTR为70 bp，带有多聚（A）尾巴。启动子活性最高发生在调节区（–3000至–2400 bp）。Gas2-GFP融合蛋白分布在细胞质内。定量逆转录聚合酶链反应和免疫印迹分析表明，gas2基因在肝脏，肌肉，低温应激明显影响了大脑和大脑。gas2 RNAi的结果显示gas2和P53基因的表达降低。这些结果表明罗非鱼gas2基因可能与低温胁迫诱导的细胞凋亡有关。