Albumin is known to have neuroprotective effects. The protein has a long half-life circulation, and its effects can therefore persist for a long time to aid in the recovery of brain ischemia. In the present study, we investigated the neuroprotective effects of human serum albumin (HSA) on brain hemodynamics. Albumin is administrated using repeated oral gavage to the rodents. Sprague-Dawley rats underwent middle cerebral artery occlusion procedures and served as a stroke model. Afterwards, 25% human serum albumin (1.25 g/kg) or saline (5 ml/kg) was orally administrated for 2 weeks in alternating days. After 2 weeks, the rodents were assessed for levels of brain ischemia. Our testing battery consists of behavioral tests and in vivo optical imaging sessions. Modified neurological severity scores (mNSS) were obtained to assess the levels of ischemia and the effects of HSA oral administration. We found that the experimental group demonstrated larger hemodynamic responses following sensory stimulation than controls that were administered with saline. HSA administration resulted in more significant changes in cerebral blood volume following direct cortical electric stimulation. In addition, the mNSS of the treatment group was lower than the control group. In particular, brain tissue staining revealed that the infarct size was also much smaller with HSA administration. This study provides support for the efficacy of HSA, and that long-term oral administration of HSA may induce neuroprotective effects against brain ischemia.

中文翻译：

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反复口服人血清白蛋白可预防MCAO后大鼠脑缺血。

已知白蛋白具有神经保护作用。该蛋白具有长的半衰期循环，因此其作用可以持续很长时间，以帮助脑缺血的恢复。在本研究中，我们调查了人血清白蛋白（HSA）对脑血流动力学的神经保护作用。使用白蛋白反复口服管饲啮齿动物。Sprague-Dawley大鼠接受大脑中动脉闭塞手术，并作为中风模型。之后，每隔一天口服25％的人血清白蛋白（1.25 g / kg）或生理盐水（5 ml / kg）。2周后，评估啮齿动物的脑缺血水平。我们的测试电池包括行为测试和体内测试光学成像会议。获得改良的神经系统严重程度评分（mNSS）以评估局部缺血水平和口服HSA的效果。我们发现实验组在感觉刺激后表现出比生理盐水对照组更大的血液动力学反应。在直接皮层电刺激后，HSA给药导致脑血容量发生更显着的变化。另外，治疗组的mNSS低于对照组。特别是，脑组织染色显示，使用HSA时，梗塞面积也小得多。这项研究为HSA的功效提供了支持，长期口服HSA可能会诱导针对脑缺血的神经保护作用。