Hybrid rice has contributed significantly to the world food security. Breeding of elite high-yield, strong-resistant broad-spectrum restorer line is an important strategy for hybrid rice in commercial breeding programs. Here, we developed three elite brown planthopper (BPH)-resistant wide-spectrum restorer lines by pyramiding big-panicle gene Gn8.1, BPH-resistant genes Bph6 and Bph9, fertility restorer genes Rf3, Rf4, Rf5, and Rf6 through molecular marker assisted selection. Resistance analysis revealed that the newly developed restorer lines showed stronger BPH-resistance than any of the single-gene donor parent Luoyang-6 and Luoyang-9. Moreover, the three new restorer lines had broad spectrum recovery capabilities for Honglian CMS, Wild abortive CMS and two-line GMS sterile lines, and higher grain yields than that of the recurrent parent 9,311 under nature field conditions. Importantly, the hybrid crosses also showed good performance for grain yield and BPH-resistance. Thus, the development of elite BPH-resistant wide-spectrum restorer lines has a promising future for breeding of broad spectrum BPH-resistant high-yield varieties.

中文翻译：

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三和两系杂交水稻抗BPH的优良广谱恢复系的开发。

杂交稻为世界粮食安全做出了重要贡献。优良高产，强抗性广谱恢复系的育种是杂交水稻在商业育种计划中的重要策略。在这里，我们通过分子标记将大穗基因Gn8.1，抗BPH基因Bph6和Bph9，育性恢复基因Rf3，Rf4，Rf5和Rf6金字塔化，开发了三种抗褐飞虱的广谱恢复系辅助选择。耐药性分析显示，新开发的恢复系显示出比任何单基因供体亲本洛阳6号和洛阳9号都更强的BPH抗性。此外，这三个新的恢复系具有红莲CMS，野生流产CMS和两系GMS不育系的广谱恢复能力，并且谷物产量高于轮回亲本9，311在自然野外条件下。重要的是，杂种杂交还表现出良好的谷物产量和抗BPH的性能。因此，优良的抗BPH广谱恢复系的开发对于广谱抗BPH的高产品种的育种具有广阔的前景。