Outbreaks of infections have emphasized the necessity for rapid and economic detection methods for pathogens in samples ranging from those of clinical origin to food products during production and retail storage, and increasingly, in environmental samples. Flow cytometry (FCM) allows the rapid acquisition of multi-parametric data regarding cell populations within fluidised samples. However, the application of FCM to pathogen detection depends on the availability of specific fluorescent probes such as antibodies and RNA probes capable of detecting and isolating pathogens from these diverse samples. A particular issue for FCM methodology is the ability to recover and discriminate bacteria from the sample matrix which may pose a major technical hurdle towards accurate and sensitive analysis. This review article focuses on detection of pathogens using FCM in samples originating from food, water, environmental and clinical sources and outlines the current state of the art and potential future applications.

中文翻译：

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流式细胞仪在病原菌检测中的应用。

感染的爆发强调了对从临床来源的样本到生产和零售储存期间的食品以及越来越多的环境样本中的病原体进行快速和经济的检测方法的必要性。流式细胞术 (FCM) 允许快速获取有关流化样品中细胞群的多参数数据。然而，FCM 在病原体检测中的应用取决于特定荧光探针的可用性，例如能够从这些不同样本中检测和分离病原体的抗体和 RNA 探针。FCM 方法的一个特殊问题是从样品基质中恢复和区分细菌的能力，这可能是实现准确和灵敏分析的主要技术障碍。