The current methods for melanocyte delivery to depigmented skin are invasive and often require sophisticated approaches. Here, we describe a promising simple and minimally invasive technique based on the dermarolling system. The technique involves preparation of a keratinocyte/melanocyte suspension prepared by trypsinization from a non-lesioned part of a patient's scalp skin and transepidermal delivery using a dermaroller equipped with 0.2-mm needles. Dermarolling leads to epidermal microinjuries without, however, causing pain or inflammation. The technique was applied to facial depigmented areas of five patients with stable vitiligo that were resistant to conventional UV/tacrolimus treatment. The efficacy of cell delivery was analysed histologically on punch biopsies, and repigmentation was evaluated photographically and by clinical assessment. We found that the transplanted keratinocytes rapidly degenerated, leading to keratinization, but melanocytes survived and integrated appropriately into the basal layer of the epidermis, leading to excellent repigmentation after 6 months in three cases and mild repigmentation in two cases. Although based on a small number of patients, we anticipate this technique to become a valid addition to the arsenal of therapeutic approaches to repigmentation in depigmenting disorders.

中文翻译：

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在白癜风中使用皮辊系统对未培养的表皮悬液进行首次表皮移植：顺序组织学和临床研究。

当前用于将黑素细胞递送至色素沉着的皮肤的方法是侵入性的，并且通常需要复杂的方法。在这里，我们描述了一种有前途的基于皮肤滚动系统的简单且微创技术。该技术涉及制备角质形成细胞/黑素细胞悬液，该悬液是通过胰蛋白酶消化从患者头皮的非病变部位制备的，并使用配备有0.2毫米针头的dermaroller经皮递送。真皮粉碎导致表皮微损伤，但是不会引起疼痛或发炎。该技术被应用于五名稳定的白癜风患者的面部色素沉着区域，这些患者对常规的紫外线/他克莫司治疗有抵抗力。通过穿孔活检组织学分析了细胞递送的功效，通过照相和临床评估对色素沉着进行评估。我们发现，移植的角质形成细胞迅速退化，导致角质化，但黑素细胞存活并适当地整合到表皮的基底层中，其中3例在6个月后获得了出色的色素沉着，而2例则导致了轻度的色素沉着。尽管基于少数患者，但我们预计该技术将成为色素沉着障碍中色素沉着治疗方法的有效补充。