In order to confirm the relationship between glutathione-peroxidase (GPx1) and biological significance on steroidogenesis, we have studied the immunocytochemical localization of GPx1 in the rat adrenal cortical cells. GPx1 was observed not only in cytoplasm (cytosol GPx1) but in mitochondria (mitochondrial GPx1). The staining intensity was altered by the functional state of the adrenal cortical cells. Furthermore, cytosol- and mitochondrial-GPx1 was modified by lipoperoxidative damage in the adrenal cortical cells. Therefore, we proposed that the pattern of GPx1 staining should be a more sensitive and specific indicator of oxidative damage in cells. Thus, the staining pattern of GPx1 is thought to be a useful marker for lipid peroxidation in the adrenal cortical cells.

中文翻译：

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谷胱甘肽过氧化物酶（GPx1）在大鼠肾上腺皮质中的免疫定位：类固醇生成和脂质过氧化之间的相关性。

为了确认谷胱甘肽过氧化物酶（GPx1）与类固醇生成生物学意义之间的关系，我们研究了GPx1在大鼠肾上腺皮质细胞中的免疫细胞化学定位。GPx1不仅在细胞质（胞浆GPx1）中，而且在线粒体（线粒体GPx1）中也观察到。染色强度被肾上腺皮质细胞的功能状态改变。此外，肾上腺皮质细胞中的脂过氧化损伤修饰了细胞质和线粒体GPx1。因此，我们建议GPx1染色的模式应该是细胞氧化损伤的更敏感和更具体的指标。因此，GPx1的染色模式被认为是肾上腺皮质细胞脂质过氧化的有用标记。