BACKGROUND TER measurements across confluent cellular monolayers provide a useful indication of TJ strength between epithelial and endothelial cells in culture. Having a reliable and accurate method of measuring cell-to-cell adhesion is critical to studies in pathophysiology and cancer metastasis. However, the use of different technical approaches to measure TER has reportedly yielded inconsistent measurements within the same cell lines. METHODS In the current study, we compared the peak TER values for the MDCK (canine kidney) and MCF-7 (human breast cancer) epithelial cell lines using two common approaches (Chopstick and Endohm) and two types of polymer inserts (PC and PET). RESULTS Both cell lines demonstrated a statistically significant difference in the peak TERs obtained using the two different approaches. Further, the MDCK (but not the MCF-7) cells demonstrated a statistically significant difference between the peak TERs when using the same approach but different inserts. CONCLUSION Our study indicates the importance of using a single approach when seeking to measure and compare the TER values of cultured cell lines.

中文翻译：

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经上皮电阻测量技术的比较：筷子vs. Endohm。

背景技术跨汇合的细胞单层的TER测量提供了培养中上皮细胞与内皮细胞之间的TJ强度的有用指示。拥有可靠而准确的测量细胞间粘附的方法对于病理生理学和癌症转移的研究至关重要。但是，据报道，使用不同的技术方法测量TER导致在同一细胞系中的测量结果不一致。方法在本研究中，我们使用两种常见方法（筷子和Endohm）和两种类型的聚合物插入物（PC和PET）比较了MDCK（犬肾）和MCF-7（人乳腺癌）上皮细胞系的TER峰值）。结果两种细胞系在使用两种不同方法获得的峰值TERs上均显示出统计学上的显着差异。进一步，当使用相同的方法但使用不同的插入片段时，MDCK（但不是MCF-7）细胞在峰TER之间显示出统计学上的显着差异。结论我们的研究表明在试图测量和比较培养细胞系的TER值时使用单一方法的重要性。