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Afadin controls cell polarization and mitotic spindle orientation in developing cortical radial glia.
Neural Development ( IF 3.6 ) Pub Date : 2017-05-10 , DOI: 10.1186/s13064-017-0085-2
Jennifer Rakotomamonjy 1 , Molly Brunner 2 , Christoph Jüschke 3 , Keling Zang 2 , Eric J Huang 4 , Louis F Reichardt 2 , Anjen Chenn 1
Affiliation  

BACKGROUND In developing tissues, cell polarity and tissue architecture play essential roles in the regulation of proliferation and differentiation. During cerebral cortical development, adherens junctions link highly polarized radial glial cells in a neurogenic niche that controls their behavior. How adherens junctions regulate radial glial cell polarity and/or differentiation in mammalian cortical development is poorly understood. RESULTS Conditional deletion of Afadin, a protein required for formation and maintenance of epithelial tissues, leads to abnormalities in radial glial cell polarity and subsequent loss of adherens junctions. We observed increased numbers of obliquely-oriented progenitor cell divisions, increased exit from the ventricular zone neuroepithelium, and increased production of intermediate progenitors. CONCLUSIONS Together, these findings indicate that Afadin plays an essential role in regulating apical-basal polarity and adherens junction integrity of radial glial cells, and suggest that epithelial architecture plays an important role in radial glial identity by regulating mitotic orientation and preventing premature exit from the neurogenic niche.

中文翻译:

Afadin控制发育中的皮质radial状神经胶质细胞的极化和有丝分裂纺锤体定向。

背景技术在发育中的组织中,细胞极性和组织结构在调节增殖和分化中起重要作用。在大脑皮层发育期间,粘附连接在控制其行为的神经源性利基中连接高度极化的放射状胶质细胞。粘附连接如何调节哺乳动物胶质发育中的径向胶质细胞极性和/或分化尚不清楚。结果有条件地删除了Afadin(形成和维持上皮组织所需的一种蛋白质)会导致radial神经胶质细胞极性异常,并随后失去粘附连接。我们观察到倾斜定向祖细胞分裂的数量增加,从心室区神经上皮的出口增加,中间祖细胞的产量增加。结论在一起,
更新日期:2019-11-01
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