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Eukaryotic diversity in late Pleistocene marine sediments around a shallow methane hydrate deposit in the Japan Sea.
Geobiology ( IF 3.7 ) Pub Date : 2017-04-23 , DOI: 10.1111/gbi.12233 M Kouduka 1 , A S Tanabe 2 , S Yamamoto 3 , K Yanagawa 4 , Y Nakamura 5 , F Akiba 6 , H Tomaru 7 , H Toju 8 , Y Suzuki 1
Geobiology ( IF 3.7 ) Pub Date : 2017-04-23 , DOI: 10.1111/gbi.12233 M Kouduka 1 , A S Tanabe 2 , S Yamamoto 3 , K Yanagawa 4 , Y Nakamura 5 , F Akiba 6 , H Tomaru 7 , H Toju 8 , Y Suzuki 1
Affiliation
Marine sediments contain eukaryotic DNA deposited from overlying water columns. However, a large proportion of deposited eukaryotic DNA is aerobically biodegraded in shallow marine sediments. Cold seep sediments are often anaerobic near the sediment–water interface, so eukaryotic DNA in such sediments is expected to be preserved. We investigated deeply buried marine sediments in the Japan Sea, where a methane hydrate deposit is associated with cold seeps. Quantitative PCR analysis revealed the reproducible recovery of eukaryotic DNA in marine sediments at depths up to 31.0 m in the vicinity of the methane hydrate deposit. In contrast, the reproducible recovery of eukaryotic DNA was limited to a shallow depth (8.3 m) in marine sediments not adjacent to the methane hydrate deposit in the same area. Pyrosequencing of an 18S rRNA gene variable region generated 1,276–3,307 reads per sample, which was sufficient to cover the biodiversity based on rarefaction curves. Phylogenetic analysis revealed that most of the eukaryotic DNA originated from radiolarian genera of the class Chaunacanthida, which have SrSO4 skeletons, the sea grass genus Zostera, and the seaweed genus Sargassum. Eukaryotic DNA originating from other planktonic fauna and land plants was also detected. Diatom sequences closely related to Thalassiosira spp., indicative of cold climates, were obtained from sediments deposited during the last glacial period (MIS‐2). Plant sequences of the genera Alnus, Micromonas, and Ulmus were found in sediments deposited during the warm interstadial period (MIS‐3). These results suggest the long‐term persistence of eukaryotic DNA from terrestrial and aquatic sources in marine sediments associated with cold seeps, and that the genetic information from eukaryotic DNA from deeply buried marine sediments associated with cold seeps can be used to reconstruct environments and ecosystems from the past.
中文翻译:
日本海浅层甲烷水合物沉积附近晚更新世海洋沉积物中的真核生物多样性。
海洋沉积物含有从上覆水柱沉积的真核DNA。然而,大部分沉积的真核DNA在浅海沉积物中被需氧生物降解。冷渗沉积物通常在沉积物与水的界面附近是厌氧的,因此此类沉积物中的真核DNA有望得到保存。我们调查了日本海中深埋的海洋沉积物,其中甲烷水合物沉积与冷渗漏有关。定量PCR分析显示,在甲烷水合物沉积物附近31.0 m深度的海洋沉积物中,真核DNA可再现地回收。相反,在与该地区甲烷水合物沉积物不相邻的海洋沉积物中,真核DNA的可再现回收仅限于浅深度(8.3 m)。18S rRNA基因可变区的焦磷酸测序每个样品可产生1,276–3,307个读数,这足以覆盖基于稀疏曲线的生物多样性。系统发育分析表明,大多数真核DNA来源于Chaunacanthida类的放射虫属,具有SrSO4个骨骼,即海草属Zostera和海藻属Sargassum。还检测到源自其他浮游动植物和陆地植物的真核DNA。从最后一次冰川期(MIS-2)的沉积物中获得了与Thalassiosira spp。密切相关的硅藻序列,表明寒冷的气候。Alnus,Micromonas和Ulmus属的植物序列在暖际期(MIS-3)沉积的沉积物中发现。这些结果表明,与冷渗漏相关的海洋沉积物中陆生和水生来源的真核DNA可以长期存在,而与冷渗漏相关的深埋海洋沉积物中的真核DNA的遗传信息可用于重建环境和生态系统。过去。
更新日期:2017-04-23
中文翻译:
日本海浅层甲烷水合物沉积附近晚更新世海洋沉积物中的真核生物多样性。
海洋沉积物含有从上覆水柱沉积的真核DNA。然而,大部分沉积的真核DNA在浅海沉积物中被需氧生物降解。冷渗沉积物通常在沉积物与水的界面附近是厌氧的,因此此类沉积物中的真核DNA有望得到保存。我们调查了日本海中深埋的海洋沉积物,其中甲烷水合物沉积与冷渗漏有关。定量PCR分析显示,在甲烷水合物沉积物附近31.0 m深度的海洋沉积物中,真核DNA可再现地回收。相反,在与该地区甲烷水合物沉积物不相邻的海洋沉积物中,真核DNA的可再现回收仅限于浅深度(8.3 m)。18S rRNA基因可变区的焦磷酸测序每个样品可产生1,276–3,307个读数,这足以覆盖基于稀疏曲线的生物多样性。系统发育分析表明,大多数真核DNA来源于Chaunacanthida类的放射虫属,具有SrSO4个骨骼,即海草属Zostera和海藻属Sargassum。还检测到源自其他浮游动植物和陆地植物的真核DNA。从最后一次冰川期(MIS-2)的沉积物中获得了与Thalassiosira spp。密切相关的硅藻序列,表明寒冷的气候。Alnus,Micromonas和Ulmus属的植物序列在暖际期(MIS-3)沉积的沉积物中发现。这些结果表明,与冷渗漏相关的海洋沉积物中陆生和水生来源的真核DNA可以长期存在,而与冷渗漏相关的深埋海洋沉积物中的真核DNA的遗传信息可用于重建环境和生态系统。过去。