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Estrogen Accelerates Cell Proliferation through Estrogen Receptor α during Rat Liver Regeneration after Partial Hepatectomy.
Acta Histochemica et Cytochemica ( IF 2.4 ) Pub Date : 2017-04-08 , DOI: 10.1267/ahc.17003
Baatarsuren Batmunkh 1 , Narantsog Choijookhuu 1 , Naparee Srisowanna 1 , Uugantsetseg Byambatsogt 1 , Phyu Synn Oo 1 , Mohmand Noor Ali 1 , Yuya Yamaguchi 1 , Yoshitaka Hishikawa 1
Affiliation  

Although estrogen is implicated in the regulation of cell growth and differentiation in many organs, the exact mechanism for liver regeneration is not completely understood. We investigated the effect of estrogen on liver regeneration in male and female Wistar rats after 70% partial hepatectomy (PHx) and performed immunohistochemistry, western blotting and Southwestern histochemistry. 17β-estradiol (E2) and ICI 182,780 were injected into male rats on the day before PHx. The proliferating cell nuclear antigen (PCNA) labeling index reached a maximum at 48 hr after PHx in males, and at 36 hr in females and E2-treated male rats. Estrogen receptor α (ERα) was expressed in zones 1 and 2 in male rats, but was found in all zones in female rats. Interestingly, ERα was not detected at 6-12 hr after PHx but was found at 24-168 hr in male rats. However, ERα expression was found at all sampling time-points in female and E2-treated male rats. The activity of estrogen responsive element binding proteins was detected from 12 hr after PHx in male rats but was found from 6 hr in female and E2-treated male rats. ERα was co-expressed with PCNA during liver regeneration. These results indicate that estrogen may play an important role in liver regeneration through ERα.

中文翻译:

在部分肝切除术后大鼠肝再生过程中,雌激素通过雌激素受体α促进细胞增殖。

尽管雌激素与许多器官的细胞生长和分化调控有关,但肝脏再生的确切机制尚未完全明了。我们调查了雌激素对70%部分肝切除(PHx)后雄性和雌性Wistar大鼠肝再生的影响,并进行了免疫组织化学,蛋白质印迹和西南组织化学。在PHx前一天将17β-雌二醇(E2)和ICI 182,780注入雄性大鼠。雄性PHx后48小时,雌性和经E2处理的雄性大鼠中,增殖细胞核抗原(PCNA)标记指数达到最大值。雌激素受体α(ERα)在雄性大鼠的1区和2区表达,但在雌性大鼠的所有区均发现。有趣的是,在雄性大鼠中,PHx后6-12小时未检测到ERα,但在24-168小时发现了ERα。然而,在雌性和E2处理的雄性大鼠的所有采样时间点均发现ERα表达。从雄性大鼠PHx后12小时开始检测雌激素反应性元素结合蛋白的活性,而在雌性和E2处理的雄性大鼠中从6小时开始发现。ERα在肝再生过程中与PCNA共表达。这些结果表明,雌激素可能通过ERα在肝脏再生中起重要作用。
更新日期:2019-11-01
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