Recently a great interest in the field of protein engineering and the design of innovative drug delivery systems employing specific ligands such as cyclodextrins is observed. The paper reports the solid state, thermal method for protein coupling with β-cyclodextrin and the physicochemical and biological properties of the obtained conjugates. The structure of the obtained conjugates was investigated via liquid chromatography-mass spectrometry, dynamic light scattering and circular dichroism analysis. The presented conjugates were biologically active and covalently bound β-cyclodextrin preserved the ability to form inclusion complexes with the model compound. This report demonstrates the great potential of cyclodextrin as a modifying unit that can be used to modulate the properties of therapeutic proteins, additionally giving such conjugates the possibility to transport many therapeutic substances in the form of inclusion complexes. In addition, the paper presents the potential of protein-cyclodextrin conjugates to construct innovative bioactive molecules for biological and medical applications.

中文翻译：

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β-环糊精-溶菌酶偶联物的合成及其理化和生化特性

最近，人们对蛋白质工程领域和使用特定配体（如环糊精）的创新药物递送系统的设计产生了浓厚的兴趣。该论文报告了蛋白质与 β-环糊精偶联的固态、热方法以及所得偶联物的理化和生物学特性。通过液相色谱-质谱法、动态光散射和圆二色性分析研究了所得缀合物的结构。所提出的偶联物具有生物活性，共价结合的 β-环糊精保留了与模型化合物形成包合物的能力。这份报告证明了环糊精作为修饰单元的巨大潜力，可用于调节治疗性蛋白质的特性，此外，使这种结合物能够以包合物的形式运输许多治疗物质。此外，该论文展示了蛋白质-环糊精偶联物在构建用于生物和医学应用的创新生物活性分子方面的潜力。