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Dynamic metabolic responses of brown planthoppers towards susceptible and resistant rice plants.
Plant Biotechnology Journal ( IF 13.8 ) Pub Date : 2017-03-10 , DOI: 10.1111/pbi.12721
Caixiang Liu 1 , Ba Du 2 , Fuhua Hao 1 , Hehua Lei 1 , Qianfen Wan 1, 3 , Guangcun He 2 , Yulan Wang 1, 4 , Huiru Tang 1, 3
Affiliation  

Brown planthopper (Nilaparvata lugens Stål, BPH) causes huge economic losses in rice-growing regions, and new strategies for combating BPH are required. To understand how BPHs respond towards BPH-resistant plants, we systematically analysed the metabolic differences between BPHs feeding on the resistant and susceptible plants using NMR and GC-FID/MS. We also measured the expression of some related genes involving glycolysis and biosyntheses of trehalose, amino acids, chitin and fatty acids using real-time PCR. BPH metabonome was dominated by more than 60 metabolites including fatty acids, amino acids, carbohydrates, nucleosides/nucleotides and TCA cycle intermediates. After initial 12 h, BPHs feeding on the resistant plants had lower levels of amino acids, glucose, fatty acids and TCA cycle intermediates than on the susceptible ones. The levels of these metabolites recovered after 24 h feeding. This accompanied with increased level in trehalose, choline metabolites and nucleosides/nucleotides compared with BPH feeding on the susceptible plants. Decreased levels of BPH metabolites at the early feeding probably resulted from less BPH uptakes of sap from resistant plants and recovery of BPH metabolites at the later stage probably resulted from their adaptation to the adverse environment with their increased hopping frequency to ingest more sap together with contributions from yeast-like symbionts in BPHs. Throughout 96 h, BPH feeding on the resistant plants showed significant up-regulation of chitin synthase catalysing biosynthesis of chitin for insect exoskeleton, peritrophic membrane lining gut and tracheae. These findings provided useful metabolic information for understanding the BPH-rice interactions and perhaps for developing new BPH-combating strategies.

中文翻译:

褐飞虱对易感和抗性水稻植株的动态代谢反应。

褐飞虱(Nilaparvata lugensStål,BPH)在水稻种植地区造成巨大的经济损失,因此需要新的应对BPH的策略。为了了解BPH对BPH抗性植物的反应,我们使用NMR和GC-FID / MS系统分析了以抗性和易感植物为食的BPH之间的代谢差异。我们还使用实时PCR测量了涉及糖酵解和海藻糖,氨基酸,几丁质和脂肪酸生物合成的一些相关基因的表达。BPH代谢组主要由60多种代谢产物控制,包括脂肪酸,氨基酸,碳水化合物,核苷/核苷酸和TCA循环中间体。在最初的12小时后,以抗性植物为食的BPHs的氨基酸,葡萄糖,脂肪酸和TCA循环中间体的含量低于易感植物。喂食24小时后,这些代谢物的水平得以恢复。与饲喂易感植物的BPH相比,海藻糖,胆碱代谢产物和核苷/核苷酸的含量增加。早期进食中BPH代谢物水平降低可能是由于抗性植物对BPH的吸收减少,而BPH代谢物在后期的恢复则可能是由于它们适应不利环境的原因,因为它们增加了跳跃频率以摄取更多的汁液并做出了贡献来自BPHs中的酵母样共生体。在整个96小时内,以抗性植物为食的BPH表现出显着上调的甲壳素合酶催化甲壳素的生物合成,以用于昆虫外骨骼,营养膜衬里的肠道和气管。
更新日期:2017-04-13
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