Diet profoundly influences the behavior of animals across many phyla. Despite this, most laboratories using model organisms, such as Drosophila, use multiple, different, commercial or custom-made media for rearing their animals. In addition to measuring growth, fecundity and longevity, we used several behavioral and physiological assays to determine if and how altering food media influence wild-type (Canton S) Drosophila melanogaster, at larval, pupal, and adult stages. Comparing 2 commonly used commercial food media we observed several key developmental and morphological differences. Third-instar larvae and pupae developmental timing, body weight and size, and even lifespan significantly differed between the 2 diets, and some of these differences persisted into adulthood. Diet was also found to produce significantly different thermal preference, locomotory capacity for geotaxis, feeding rates, and lower muscle response to hormonal stimulation. There were no differences, however, in adult thermal preferences, in the number or viability of eggs laid, or in olfactory learning and memory between the diets. We characterized the composition of the 2 diets and found particularly significant differences in cholesterol and (phospho)lipids between them. Notably, diacylglycerol (DAG) concentrations vary substantially between the 2 diets, and may contribute to key phenotypic differences, including lifespan. Overall, the data confirm that 2 different diets can profoundly influence the behavior, physiology, morphology and development of wild-type Drosophila, with greater behavioral and physiologic differences occurring during the larval stages.

饮食深刻影响了许多门上动物的行为。尽管如此，大多数使用模型生物（如果蝇）的实验室还是使用多种不同的商业或定制培养基饲养动物。除了测量生长，繁殖力和寿命外，我们还使用了几种行为和生理分析方法来确定改变食物介质是否以及如何影响野生型（S州）果蝇（Drosophila melanogaster），处于幼虫期，up期和成年期。比较2种常用的商业食品介质，我们观察到了几个关键的发育和形态差异。两种饮食在三龄幼虫和and的发育时间，体重和大小，甚至寿命上都存在显着差异，其中一些差异一直持续到成年期。还发现饮食产生明显不同的热偏好，趋向性的运动能力，进食速率以及对激素刺激的较低肌肉反应。但是，在成年后的喜好，产卵的数量或生存力或两种饮食之间的嗅觉学习和记忆方面没有差异。我们表征了这两种饮食的组成，发现它们之间的胆固醇和（磷脂）脂质特别显着不同。尤其，两种饮食之间的二酰基甘油（DAG）浓度差异很大，并且可能导致关键的表型差异，包括寿命。总体而言，数据证实两种不同的饮食可以深刻影响野生型动物的行为，生理，形态和发育果蝇，在幼虫阶段有较大的行为和生理差异。