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Genetic mapping and QTL analysis of Botrytis resistance in Gerbera hybrida.
Molecular Breeding ( IF 3.1 ) Pub Date : 2017-02-22 , DOI: 10.1007/s11032-016-0617-1
Yiqian Fu 1 , Alex van Silfhout 1 , Arwa Shahin 1 , Ronny Egberts 2 , Martin Beers 3 , Ans van der Velde 3 , Adrie van Houten 2 , Jaap M van Tuyl 1 , Richard G F Visser 1 , Paul Arens 1
Affiliation  

Gerbera hybrida is an economically important cut flower. In the production and transportation of gerbera with unavoidable periods of high relative humidity, grey mould occurs and results in losses in quality and quantity of flowers. Considering the limitations of chemical use in greenhouses and the impossibility to use these chemicals in auction or after sale, breeding for resistant gerbera cultivars is considered as the best practical approach. In this study, we developed two segregating F1 populations (called S and F). Four parental linkage maps were constructed using common and parental specific SNP markers developed from expressed sequence tag sequencing. Parental genetic maps, containing 30, 29, 27 and 28 linkage groups and a consensus map covering 24 of the 25 expected chromosomes, could be constructed. After evaluation of Botrytis disease severity using three different tests, whole inflorescence, bottom (of disc florets) and ray floret, quantitative trait locus (QTL) mapping was performed using the four individual parental maps. A total of 20 QTLs (including one identical QTL for whole inflorescence and bottom tests) were identified in the parental maps of the two populations. The number of QTLs found and the explained variance of most QTLs detected reflect the complex mechanism of Botrytis disease response.

中文翻译:

非洲菊杂种中灰霉病抗性的遗传图谱和QTL分析。

非洲菊杂种是经济上重要的切花。在非洲菊的生产和运输过程中,不可避免地会出现较高的相对湿度,因此会出现灰霉病,并导致花卉质量和数量的损失。考虑到温室中化学药品使用的局限性以及无法在拍卖或售后中使用这些化学药品,抗性非洲菊品种的育种被认为是最佳的实用方法。在这项研究中,我们开发了两个隔离的F1种群(称为S和F)。使用从表达的序列标签测序开发的常见和亲本特异性SNP标记构建了四个亲本连锁图谱。可以构建包含30、29、27和28个连接基团的亲本遗传图谱,以及涵盖25个预期染色体中的24条的共识图谱。在使用三种不同的测试方法(整个花序,底部(盘状小花的)和射线小花)评估葡萄孢病的严重性后,使用四个单独的亲本图谱进行了数量性状基因座(QTL)作图。在两个种群的亲本图中,总共鉴定出20个QTL(包括用于完整花序和底部试验的一个相同的QTL)。发现的QTL数量和大多数检测到的QTL的解释方差反映了葡萄孢菌疾病反应的复杂机制。
更新日期:2019-11-01
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