Eight cultivars of Brassica oleracea var. capitata and two types of explant (hypocotyl and cotyledon) were tested for their potential to regenerate under in vitro conditions. Hypocotyl and cotyledon explants from 10-d-old seedlings were subcultured onto different callus induction media based on Murashige and Skoog (MS) basal medium supplemented with 1% sucrose and different concentrations and combinations of plant growth regulators. Hypocotyl explants were found to be more suitable for callus induction and organogenesis than cotyledon explants for all cultivars tested. In terms of regeneration, the cv. 'Amager' was significantly more responsive than the other cultivars tested and produced the highest number of shoots/buds per explant. Moreover, among five types of media tested, MS + 8.88 μM 6-benzyloaminopurine (BAP) + 0.53 μM α-naphthylacetic acid (NAA) was most effective for shoot regeneration. Rooting was achieved within 10-15 d on all the rooting media, but MS medium containing 5.37 μM NAA produced the maximum number of strong and healthy roots. Plantlets (95%) were subsequently established in the greenhouse, and no phenotypic variations were observed among regenerated plants. This plant regeneration protocol could be suitable for a wide range of cabbage cultivars.

中文翻译：

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甘蓝变种8个品种的离体再生。卡皮塔塔。

甘蓝变种八个品种。测试了人参和两种外植体（下胚轴和子叶）在体外条件下的再生潜力。将来自10 d龄幼苗的下胚轴和子叶外植体传代培养到基于Murashige和Skoog（MS）基础培养基的不同愈伤组织诱导培养基上，该培养基补充了1％蔗糖和不同浓度的植物生长调节剂和组合。发现对于所有测试的品种，下胚轴外植体比子叶外植体更适合于愈伤组织的诱导和器官发生。在再生方面，简历。与其他测试品种相比，'Amager'的反应显着增强，并且每个外植体产生的芽/芽数量最多。此外，在所测试的五种介质中，MS + 8.88μM6-苄基氨基嘌呤（BAP）+ 0。53μMα-萘乙酸（NAA）对芽再生最有效。在所有生根培养基上均可在10-15 d内生根，但含有5.37μMNAA的MS培养基可产生最大数量的坚固和健康的根。随后在温室中建立了小植株（95％），并且在再生植株之间未观察到表型变异。这种植物再生方案可以适用于广泛的甘蓝品种。