In this study, optimization conditions for cyanide biodegradation by Klebsiella pneumoniae strain were determined to be 25 °C, pH=7 and 150 rpm at the concentration of 0.5 mM potassium cyanide in the medium. Additionally, it was found that K. pneumoniae strain is not only able to degrade potassium cyanide, but also to degrade potassium hexacyanoferrate(II) trihydrate and sodium ferrocyanide decahydrate with the efficiencies of 85 and 87.5%, respectively. Furthermore, this strain degraded potassium cyanide in the presence of different ions such as magnesium, nickel, cobalt, iron, chromium, arsenic and zinc, in variable concentrations (0.1, 0.25 and 0.5 mM) and as a result the amount of the bacteria in the biodegradation media decreased with the increase of ion concentration. Lastly, it was also observed that sterile crude extract of K. pneumoniae strain degraded potassium cyanide on the fifth day of incubation. Based on these results, it is concluded that both culture and sterile crude extract of K. pnemoniae will be used in cyanide removal from different wastes.

中文翻译：

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使用肺炎克雷伯菌的生物处理氰化物。

在这项研究中，确定在培养基中浓度为0.5 mM氰化钾的条件下，肺炎克雷伯菌的生物降解氰化物的最佳条件为25°C，pH = 7和150 rpm。另外，发现肺炎克雷伯菌该菌株不仅能够降解氰化钾，而且能够降解三水合六氰合铁酸钾（II）和十水合亚铁氰化钠，效率分别为85％和87.5％。此外，该菌株在不同浓度（0.1、0.25和0.5 mM）的镁，镍，钴，铁，铬，砷和锌等不同离子的存在下降解了氰化钾，结果细菌的数量增加了。生物降解介质随着离子浓度的增加而降低。最后，还观察到在培养的第五天，肺炎克雷伯菌菌株的无菌粗提物降解了氰化钾。根据这些结果，可以得出结论：假单胞菌的培养物和无菌粗提物均如此 将用于从不同废物中去除氰化物。