BACKGROUND Indole-3-acetic acid (IAA) extraction and purification are of great importance in auxin research, which is a hot topic in the plant growth and development field. Solid-phase extraction (SPE) is frequently used for IAA extraction and purification. However, no IAA-specific SPE columns are commercially available at the moment. Therefore, the development of IAA-specific recognition materials and IAA extraction and purification methods will help researchers meet the need for more precise analytical methods for research on phytohormones. RESULTS Since the AUXIN RESISTANT/INDOLE-3-ACETIC ACID INDUCIBLE (Aux/IAA) proteins show higher specific binding capability with auxin, recombinant IAA1, IAA7 and IAA28 proteins were used as sorbents to develop an IAA extraction and purification method. A GST tag was used to solidify the recombinant protein in a column. Aux/IAA proteins solidified in a column have successfully trapped trace IAA in aqueous solutions. The IAA7 protein showed higher IAA binding capability than the other proteins tested. In addition, expression of the IAA7 protein in Drosophila Schneider 2 (S2) cells produced better levels of binding than IAA7 expressed in E. coli. CONCLUSION This work validated the potential of Aux/IAA proteins to extract and purify IAA from crude plant extracts once we refined the techniques for these processes.

中文翻译：

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基于重组Aux / IAA蛋白的生长素提取和纯化。

背景技术吲哚-3-乙酸（IAA）的提取和纯化在植物生长素研究中具有重要意义，这是植物生长发育领域的一个热门课题。固相萃取（SPE）通常用于IAA萃取和纯化。但是，目前尚无IAA专用的SPE色谱柱可商购。因此，开发IAA特异性识别材料以及IAA提取和纯化方法将有助于研究人员满足对植物激素研究需要更精确的分析方法的需求。结果由于抗AUXIN /吲哚-3-乙酸的蛋白（Aux / IAA）与生长素具有更高的特异性结合能力，因此重组IAA1，IAA7和IAA28蛋白被用作吸附剂，从而开发了IAA提取和纯化方法。使用GST标签在柱子中固化重组蛋白。在柱中固化的Aux / IAA蛋白已成功地将痕量IAA捕获在水溶液中。IAA7蛋白显示出比其他测试蛋白更高的IAA结合能力。另外，果蝇施耐德2（S2）细胞中IAA7蛋白的表达比大肠杆菌中表达的IAA7产生更好的结合水平。结论一旦我们改进了这些过程的技术，这项工作就验证了Aux / IAA蛋白从粗植物提取物中提取和纯化IAA的潜力。果蝇施奈德2（S2）细胞中IAA7蛋白的表达比大肠杆菌中表达的IAA7产生更好的结合水平。结论一旦我们改进了这些过程的技术，这项工作就验证了Aux / IAA蛋白从粗植物提取物中提取和纯化IAA的潜力。果蝇施奈德2（S2）细胞中IAA7蛋白的表达比大肠杆菌中表达的IAA7产生更好的结合水平。结论一旦我们改进了这些过程的技术，这项工作就验证了Aux / IAA蛋白从粗植物提取物中提取和纯化IAA的潜力。