Siderophores are produced by a number of microbes to capture iron with outstandingly high affinity, which property also generates biomedical and industrial interests. Desferrioxamine E (DFO-E) secreted by streptomycetes bacteria can be an ideal candidate for iron chelation therapy, which necessitates its cost-effective production for in vitro and animal studies. This study focused on the optimization of DFO-E production by Streptomyces parvulus CBS548.68. Different combinations of various carbon and nitrogen sources as well as the addition of 3-morpholinopropane-1-sulfonic acid (MOPS) markedly affected DFO-E yields, which were attributed, at least in part, to the higher biomass productions found in MOPS-supplemented cultures. In MOPS-supplemented glucose and sodium glutamate medium, DFO-E productions as high as 2,009 ± 90 mg/l of culture medium were reached. High-performance liquid chromatography analysis demonstrated that a simple two-step purification process yielded DFO-E preparations with purities of ∼97%. Matrix assisted laser desorption ionization-time of flight mass spectrometry analysis showed that purified DFO-E always contained traces of desferrioxamine D2.

中文翻译：

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小链霉菌生产去铁草胺E的最优化。

铁载体是由许多微生物产生的，以极高的亲和力捕获铁，这种特性也引起了生物医学和工业利益。链霉菌细菌分泌的去铁胺（Esferrioxamine E）（DFO-E）可能是铁螯合疗法的理想候选者，这使其有成本效益地用于体外和动物研究。这项研究集中在小链霉菌CBS548.68对DFO-E生产的优化上。各种碳源和氮源的不同组合以及3-吗啉代丙烷-1-磺酸（MOPS）的添加显着影响DFO-E的产量，这至少部分归因于MOPS-补充文化。在MOPS补充的葡萄糖和谷氨酸钠培养基中，DFO-E的产量高达2，达到009±90 mg / l的培养基。高效液相色谱分析表明，简单的两步纯化过程可制得纯度约为97％的DFO-E制剂。基质辅助激光解吸电离飞行时间质谱分析表明，纯化的DFO-E始终含有痕量去铁胺D2。