BACKGROUND The aim of the present study was to investigate the effects of Scutellaria barbata flavonoids (SBF) on memory impairment and neuronal injury induced by amyloid beta protein 25-35 in combination with aluminum trichloride (AlCl3) and recombinant human transforming growth factor-β1 (RHTGF-β1) (composited Aβ) in rats. METHODS The composited Aβ-treated model of Alzheimer's disease (AD)-like memory impairment and neuronal injury was established in male rats by right intracerebroventricular injection of composited Aβ, and the effects of SBF were assessed using this rat model. Spatial learning and memory of rats were assessed in the Morris water maze, and neuronal injury was assessed by light and electron microscopy with hematoxylin-eosin or uranyl acetate and lead nitrate-sodium citrate staining, respectively. RESULTS In the Morris water maze, memory impairment was observed in 94.7% of the composited Aβ-treated rats. The composited Aβ-treated rats took longer than sham-operated rats to find the hidden platform during position navigation and reversal learning trials. They also spent less time swimming in the target quadrant in the probe trial. Optical and electron microscopic observations showed significant neuropathological changes including neuron loss or pyknosis in hippocampus, typical colliquative necrosis in cerebral cortex, mitochondrial swelling and cristae fragmentation and a large number of lipofuscin deposits in the cytoplasm. Treatment with SBF (35-140 mg/kg) reduced the memory impairment and neuronal injury induced by composited Aβ. CONCLUSION SBF-mediated improvement of composited Aβ-induced memory impairment and neuronal injury in rats provides an appropriate rationale for evaluating SBF as a promising agent for treatment of AD.

中文翻译：

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半枝莲类黄酮减轻大鼠复合Aβ引起的记忆障碍和神经元损伤。

背景技术本研究的目的是研究半枝cut黄酮（SBF）对淀粉样蛋白25-35与三氯化铝（AlCl3）和重组人转化生长因子-β1（β-淀粉样蛋白）诱导的记忆障碍和神经元损伤的影响。 RHTGF-β1）（复合Aβ）在大鼠中。方法通过右脑室内注射复合Aβ建立雄性大鼠阿尔茨海默氏病（AD）样记忆障碍和神经元损伤的复合Aβ治疗模型，并用该大鼠模型评估SBF的作用。在莫里斯水迷宫中评估大鼠的空间学习和记忆，并分别通过苏木精-曙红或乙酸铀酰和硝酸铅-柠檬酸钠染色的光学和电子显微镜评估神经元损伤。结果在莫里斯水迷宫中，在94.7％的复合Aβ处理的大鼠中观察到记忆障碍。在位置导航和逆向学习试验中，复合Aβ处理的大鼠比假手术的大鼠需要更长的时间才能找到隐藏的平台。在探查试验中，他们还减少了在目标象限内游泳的时间。光学和电子显微镜观察显示出明显的神经病理学改变，包括海马神经元丢失或萎缩，大脑皮质典型的补语坏死，线粒体肿胀和cr裂以及细胞质中大量脂褐素沉积。SBF（35-140 mg / kg）的治疗减少了复合Aβ引起的记忆障碍和神经元损伤。